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Literature summary for 1.3.3.3 extracted from

  • Macieira, S.; Martins, B.M.; Huber, R.
    Oxygen-dependent coproporphyrinogen-III oxidase from Escherichia coli: one-step purification and biochemical characterisation (2003), FEMS Microbiol. Lett., 226, 31-37.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
Coproporphyrinogen-III recombinant wild-type enzyme, pH 7.0, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ about 0.03 mol per mol of polypeptide for both enzyme forms Escherichia coli
Fe2+ 0.09-1.25 mol per mol of polypeptide for the recombinant wild-type enzyme, 0.2 mol per mol of polypeptide for the selenomethionine-labeled variant Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 35000, recombinant enzyme, SDS-PAGE, 2 * 35390, recombinant enzyme, mass spectrometry, 2 * 35718, recombinant selenomethionine-labeled enzyme, mass spectrometry Escherichia coli
35390
-
2 * 35000, recombinant enzyme, SDS-PAGE, 2 * 35390, recombinant enzyme, mass spectrometry, 2 * 35718, recombinant selenomethionine-labeled enzyme, mass spectrometry Escherichia coli
35718
-
2 * 35000, recombinant enzyme, SDS-PAGE, 2 * 35390, recombinant enzyme, mass spectrometry, 2 * 35718, recombinant selenomethionine-labeled enzyme, mass spectrometry Escherichia coli
50000
-
recombinant enzyme, gel filtration Escherichia coli
70000
-
recombinant enzyme, native PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P36553 oxygen-dependent enzyme form HemF
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37.5
-
purified recombinant wild-type and selenomethinonine enzymes Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen-III + O2
-
Escherichia coli protoporphyrinogen-IX + CO2 + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000, recombinant enzyme, SDS-PAGE, 2 * 35390, recombinant enzyme, mass spectrometry, 2 * 35718, recombinant selenomethionine-labeled enzyme, mass spectrometry Escherichia coli
More enzyme consists of 26% alpha-helices and 31% beta-sheets Escherichia coli

Synonyms

Synonyms Comment Organism
coproporphyrinogen-III oxidase
-
Escherichia coli
CPO
-
Escherichia coli
HemF
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
additional information enzyme contains a covalently bound porphyrin compound Escherichia coli