KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
Coproporphyrinogen-III | recombinant wild-type enzyme, pH 7.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | about 0.03 mol per mol of polypeptide for both enzyme forms | Escherichia coli | |
Fe2+ | 0.09-1.25 mol per mol of polypeptide for the recombinant wild-type enzyme, 0.2 mol per mol of polypeptide for the selenomethionine-labeled variant | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, recombinant enzyme, SDS-PAGE, 2 * 35390, recombinant enzyme, mass spectrometry, 2 * 35718, recombinant selenomethionine-labeled enzyme, mass spectrometry | Escherichia coli |
35390 | - |
2 * 35000, recombinant enzyme, SDS-PAGE, 2 * 35390, recombinant enzyme, mass spectrometry, 2 * 35718, recombinant selenomethionine-labeled enzyme, mass spectrometry | Escherichia coli |
35718 | - |
2 * 35000, recombinant enzyme, SDS-PAGE, 2 * 35390, recombinant enzyme, mass spectrometry, 2 * 35718, recombinant selenomethionine-labeled enzyme, mass spectrometry | Escherichia coli |
50000 | - |
recombinant enzyme, gel filtration | Escherichia coli |
70000 | - |
recombinant enzyme, native PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P36553 | oxygen-dependent enzyme form HemF | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.5 | - |
purified recombinant wild-type and selenomethinonine enzymes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen-III + O2 | - |
Escherichia coli | protoporphyrinogen-IX + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, recombinant enzyme, SDS-PAGE, 2 * 35390, recombinant enzyme, mass spectrometry, 2 * 35718, recombinant selenomethionine-labeled enzyme, mass spectrometry | Escherichia coli |
More | enzyme consists of 26% alpha-helices and 31% beta-sheets | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
coproporphyrinogen-III oxidase | - |
Escherichia coli |
CPO | - |
Escherichia coli |
HemF | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme contains a covalently bound porphyrin compound | Escherichia coli |