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Literature summary for 1.3.3.3 extracted from

  • Camadro, J.M.; Chambon, H.; Jolles, J.; Labbe, P.
    Purification and properties of coproporphyrinogen oxidase from yeast Saccharomyces cerevisiae (1986), Eur. J. Biochem., 156, 579-587.
    View publication on PubMed
Search for more literature for 1.3.3.3

Activating Compound

Activating Compound Comment Organism Structure
lecithin 1.7fold activation Saccharomyces cerevisiae
Non-ionic detergents 2.5fold activation Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
5,5-dithiobis(2-nitrobenzoic acid) 30-40% inhibition at 1 mM Saccharomyces cerevisiae
iodoacetate 40-50% inhibition at 1 mM Saccharomyces cerevisiae
p-chloromercuribenzoate 60% inhibition at 1 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 Coproporphyrinogen-III
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharomyces cerevisiae 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 2 atoms per native molecule Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Saccharomyces cerevisiae
70000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coproporphyrinogen-III + O2 + 2 H+ Saccharomyces cerevisiae same activity under aerobic and anaerobic conditions protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Saccharomyces cerevisiae
-

Storage Stability

Storage Stability Organism
-30°C, 0.1 M potassium phosphate, pH 7.6, stable for months Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen-III + O2 + 2 H+ same activity under aerobic and anaerobic conditions Saccharomyces cerevisiae protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6.4 8.4 80% of the maximum activity within Saccharomyces cerevisiae