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Literature summary for 1.3.3.14 extracted from

  • Yoshimoto, A.; Ogasawara, T.; Kitamura, I.; Oki, T.; Inui, T.; Takeuchi, T.; Umezawa, H.
    Enzymatic conversion of aclacinomycin A to Y by a specific oxidoreductase in Streptomyces (1979), J. Antibiot., 32, 472-481.
    View publication on PubMed

General Stability

General Stability Organism
no loss of activity is observed on freezing Streptomyces galilaeus

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid 5 mM, 44% inhibition Streptomyces galilaeus
Fe2+ 0.5 mM, 26% inhibition. 1 mM, 73% inhibition Streptomyces galilaeus
NaN3 1 mM, 15% inhibition. 5 mM, 53% inhibition Streptomyces galilaeus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
gel filtration Streptomyces galilaeus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aclacinomycin A + O2 Streptomyces galilaeus
-
aclacinomycin Y + H2O2
-
?
aclacinomycin A + O2 Streptomyces galilaeus MA144-M1 6U-21
-
aclacinomycin Y + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces galilaeus
-
-
-
Streptomyces galilaeus MA144-M1 6U-21
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces galilaeus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
952
-
pH and temperature not specified in the publication Streptomyces galilaeus

Storage Stability

Storage Stability Organism
-20°C, in 10% glycerol, 0.05 M Tris-HCl, pH 7.2, stable for at least 2 months Streptomyces galilaeus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aclacinomycin A + O2
-
Streptomyces galilaeus aclacinomycin Y + H2O2
-
?
aclacinomycin A + O2 the enzyme catalyzes the oxidation of the terminal sugar, L-cinerulose, of the trisaccharide moiety of aclacinomycin A to L-aculose (2,3,6-trideoxyhex-2-enopyranos-4-ulose) with removal of two electrons. NAD+, NADP+, phenazine methosulfate and 2,6-dichlorophenolindophenol can not serve as electron acceptors Streptomyces galilaeus aclacinomycin Y + H2O2
-
?
aclacinomycin A + O2
-
Streptomyces galilaeus MA144-M1 6U-21 aclacinomycin Y + H2O2
-
?
aclacinomycin A + O2 the enzyme catalyzes the oxidation of the terminal sugar, L-cinerulose, of the trisaccharide moiety of aclacinomycin A to L-aculose (2,3,6-trideoxyhex-2-enopyranos-4-ulose) with removal of two electrons. NAD+, NADP+, phenazine methosulfate and 2,6-dichlorophenolindophenol can not serve as electron acceptors Streptomyces galilaeus MA144-M1 6U-21 aclacinomycin Y + H2O2
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 7, 30 min, stable Streptomyces galilaeus
60
-
pH 7, 30 min, 20% loss of activity Streptomyces galilaeus
80
-
pH 7, 30 min, 50% loss of activity Streptomyces galilaeus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Streptomyces galilaeus

pH Range

pH Minimum pH Maximum Comment Organism
3.8 7 pH 3.8: about 40% of maximal activity, pH 7.0: about 65% of maximal activity Streptomyces galilaeus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 37°C, 3 h, stable Streptomyces galilaeus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces galilaeus isoelectric focusing
-
5.9