Literature summary for 1.3.3.10 extracted from

Genet, R.; Benetti, P.H.; Hammadi, A.; Menez, A.
L-tryptophan 2',3'-oxidase from Chromobacterium violaceum (1995), J. Biol. Chem., 270, 23540-23545.

Search for more literature for 1.3.3.10

General Stability

General Stability	Organism
active with 0.2 M dithiothreitol, 1% SDS, or 4.5 M urea	Chromobacterium violaceum

Inhibitors

Inhibitors	Comment	Organism	Structure
5-hydroxy-L-tryptophan	competitive inhibition, Ki: 0.037 mM	Chromobacterium violaceum
D-tryptophan	competitive inhibition, Ki: 0.0066 mM	Chromobacterium violaceum
methyl-3-indole	competitive inhibition, Ki: 0.0013 mM	Chromobacterium violaceum
additional information	no inhibition with L-histidine, N-acetyl-L-phenylalaninamide and N-acetyl-L-tyrosinamide	Chromobacterium violaceum
tryptamine	competitive inhibition, Ki: 0.0054 mMl	Chromobacterium violaceum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0058	-	L-tryptophan	-	Chromobacterium violaceum
0.0091	-	L-Tryptophanamide	-	Chromobacterium violaceum
0.0195	-	N-Acetyl-L-tryptophanamide	-	Chromobacterium violaceum
0.02	-	indole-3-propionate	-	Chromobacterium violaceum
0.026	-	pentagastrin	dehydrogenation of the tryptophanyl side chain	Chromobacterium violaceum
0.071	-	Luteinizing hormone-releasing hormone	dehydrogenation of the tryptophanyl side chain	Chromobacterium violaceum
0.0791	-	N-acetyl-L-tryptophan	-	Chromobacterium violaceum
0.93	-	adrenocorticotropic hormone	dehydrogenation of the tryptophanyl side chain	Chromobacterium violaceum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
MgCl2	10 mM, residual activity 103%	Chromobacterium violaceum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14000	-	alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE	Chromobacterium violaceum
74000	-	alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE	Chromobacterium violaceum
680000	-	gel filtration	Chromobacterium violaceum

Organism

Organism	UniProt	Comment	Textmining
Chromobacterium violaceum	-	strain ATCC12472	-
Chromobacterium violaceum ATCC 12472	-	strain ATCC12472	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
30.3	-	after purification	Chromobacterium violaceum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
adrenocorticotropic hormone + O2	-	Chromobacterium violaceum	? + H2O2	-	?
indole-3-propionate + O2	-	Chromobacterium violaceum	? + H2O2	-	?
indole-3-propionate + O2	-	Chromobacterium violaceum ATCC 12472	? + H2O2	-	?
L-tryptophan + O2	-	Chromobacterium violaceum	alpha,beta-didehydrotryptophan + H2O2	-	?
L-tryptophan + O2	-	Chromobacterium violaceum ATCC 12472	alpha,beta-didehydrotryptophan + H2O2	-	?
L-tryptophanamide + O2	-	Chromobacterium violaceum	alpha,beta-didehydrotryptophanamide + H2O2	-	?
L-tryptophanamide + O2	-	Chromobacterium violaceum ATCC 12472	alpha,beta-didehydrotryptophanamide + H2O2	-	?
luteinizing hormone-releasing hormone + O2	-	Chromobacterium violaceum	? + H2O2	-	?
N-acetyl-L-tryptophan + O2	-	Chromobacterium violaceum	N-acetyl-alpha,beta-didehydrotryptophan + H2O2	-	?
N-acetyl-L-tryptophan + O2	-	Chromobacterium violaceum ATCC 12472	N-acetyl-alpha,beta-didehydrotryptophan + H2O2	-	?
N-acetyl-L-tryptophanamide + O2	-	Chromobacterium violaceum	N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2	-	?
N-acetyl-L-tryptophanamide + O2	-	Chromobacterium violaceum ATCC 12472	N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2	-	?
pentagastrin + O2	-	Chromobacterium violaceum	N-[(2E)-2-{[N-(tert-butoxycarbonyl)-beta-alanyl]amino}-3-(1H-indol-3-yl)prop-2-enoyl]-L-methionyl-L-alpha-aspartyl-L-phenylalaninamide + H2O2	-	?

Subunits

Subunits	Comment	Organism
octamer	alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE	Chromobacterium violaceum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	active up to 80øC	Chromobacterium violaceum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.283	-	adrenocorticotropic hormone	dehydrogenation of the tryptophanyl side chain	Chromobacterium violaceum
0.333	-	Luteinizing hormone-releasing hormone	dehydrogenation of the tryptophanyl side chain	Chromobacterium violaceum
0.533	-	pentagastrin	dehydrogenation of the tryptophanyl side chain	Chromobacterium violaceum
0.593	-	L-Tryptophanamide	-	Chromobacterium violaceum
0.66	-	L-tryptophan	-	Chromobacterium violaceum
0.753	-	N-Acetyl-L-tryptophanamide	-	Chromobacterium violaceum
0.785	-	indole-3-propionate	-	Chromobacterium violaceum
0.79	-	N-acetyl-L-tryptophan	-	Chromobacterium violaceum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Chromobacterium violaceum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7	-	after dilution in 0.1 M bis-Tris buffer	Chromobacterium violaceum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0013	-	methyl-3-indole	-	Chromobacterium violaceum
0.0054	-	tryptamine	-	Chromobacterium violaceum
0.0066	-	D-tryptophan	-	Chromobacterium violaceum
0.037	-	5-hydroxy-L-tryptophan	-	Chromobacterium violaceum

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Release 2023.1 (January 2023)