Cloned (Comment) | Organism |
---|---|
cDNA encoding GLDH, subcloning in Escherichia coli strain HB101, and functional expression in transgenic Nicotiana tabacum leaves and roots via Agrobacterium tumefaciens LBA4404-mediated transformation under control of the CaMV 35S promoter. Despite the elevated GLDH activity, the L-ascorbate content in the leaves does not change in all lines, but increases 6-10fold in the roots of transgenic plants, overview | Ipomoea batatas |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Ipomoea batatas | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + cytochrome c | Ipomoea batatas | - |
L-ascorbate + reduced cytochrome c | - |
? | |
additional information | Ipomoea batatas | incubation of leaf discs in a L-galactonolactone solution leads to a rapid 2 to 3fold increase in the L-ascorbate content in both GLDH-transformed and non-transformed plants in the same manner | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea batatas | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Ipomoea batatas | - |
root | - |
Ipomoea batatas | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0245 | 0.0513 | root mitochondria of transformed transgenic tobacco lines, pH not specified in the publication, temperature not specified in the publication | Ipomoea batatas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + cytochrome c | - |
Ipomoea batatas | L-ascorbate + reduced cytochrome c | - |
? | |
additional information | incubation of leaf discs in a L-galactonolactone solution leads to a rapid 2 to 3fold increase in the L-ascorbate content in both GLDH-transformed and non-transformed plants in the same manner | Ipomoea batatas | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GalL dehydrogenase | - |
Ipomoea batatas |
GLDH | - |
Ipomoea batatas |
L-galactono-1,4-lactone dehydrogenase | - |
Ipomoea batatas |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Ipomoea batatas |
General Information | Comment | Organism |
---|---|---|
metabolism | GLDH catalyzes the last step of L-ascorbate, AsA, biosynthesis in plants, but the upstream genes in the AsA biosynthetic pathway are responsible for enhancing the AsA content in plants, regulation, overview | Ipomoea batatas |