Cloned (Comment) | Organism |
---|---|
expression of MurB in Escherichia coli strain BL21(DE3) | Thermus caldophilus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant MurB, hanging drop-vapor diffusion method, 0.001 ml of protein solution containing 20 mg/ml protein in 20 mM HEPES-NaOH, pH 7.5, is mixed with 0.001 ml of reservoir solution containing 100 mM MES-NaOH, pH 6.5, 18% w/v PEG 8000 and 200 mM CaCl2, equilibration against 1 ml of mother liquor, Se-Met substituted crystals are grown under the same conditions, while the substrate-complex crystals are grown in the presence of 25 mM substrate UDPGlcNAc, crystals appear within 8 h, X-ray difraction structure determination and analysis at 1.15-1.7 A resolution | Thermus caldophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | Thermus caldophilus | - |
UDP-N-acetylmuramate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus caldophilus | Q5SJC8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant MurB from Escherichia coli strain BL21(DE3) | Thermus caldophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | - |
Thermus caldophilus | UDP-N-acetylmuramate + NADP+ | - |
r | |
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | i.e. enolpyruvyl-UDP-N-acetylglucosamine | Thermus caldophilus | UDP-N-acetylmuramate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | MurB is a type II enzyme lacking Tyr loop and split betaalphabetabeta fold | Thermus caldophilus |
Synonyms | Comment | Organism |
---|---|---|
More | a type II enzyme lacking Tyr loop and split betaalphabetabeta fold | Thermus caldophilus |
MurB | - |
Thermus caldophilus |
UDP-N-acetylenolpyruvylglucosamine reductase | - |
Thermus caldophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | binding structure, overview | Thermus caldophilus | |
NADP+ | - |
Thermus caldophilus | |
NADPH | - |
Thermus caldophilus |