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Literature summary for 1.3.1.98 extracted from

  • Kim, M.; Cho, M.K.; Song, H.; Kim, D.; Park, B.; Lee, J.H.; Kang, G.B.; Kim, S.H.; Im, Y.J.; Lee, D.; Eom, S.H.
    Crystal structure of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from Thermus caldophilus (2007), Proteins, 66, 751-754.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of MurB in Escherichia coli strain BL21(DE3) Thermus caldophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant MurB, hanging drop-vapor diffusion method, 0.001 ml of protein solution containing 20 mg/ml protein in 20 mM HEPES-NaOH, pH 7.5, is mixed with 0.001 ml of reservoir solution containing 100 mM MES-NaOH, pH 6.5, 18% w/v PEG 8000 and 200 mM CaCl2, equilibration against 1 ml of mother liquor, Se-Met substituted crystals are grown under the same conditions, while the substrate-complex crystals are grown in the presence of 25 mM substrate UDPGlcNAc, crystals appear within 8 h, X-ray difraction structure determination and analysis at 1.15-1.7 A resolution Thermus caldophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Thermus caldophilus
-
UDP-N-acetylmuramate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Thermus caldophilus Q5SJC8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant MurB from Escherichia coli strain BL21(DE3) Thermus caldophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
Thermus caldophilus UDP-N-acetylmuramate + NADP+
-
r
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH i.e. enolpyruvyl-UDP-N-acetylglucosamine Thermus caldophilus UDP-N-acetylmuramate + NADP+
-
r

Subunits

Subunits Comment Organism
More MurB is a type II enzyme lacking Tyr loop and split betaalphabetabeta fold Thermus caldophilus

Synonyms

Synonyms Comment Organism
More a type II enzyme lacking Tyr loop and split betaalphabetabeta fold Thermus caldophilus
MurB
-
Thermus caldophilus
UDP-N-acetylenolpyruvylglucosamine reductase
-
Thermus caldophilus

Cofactor

Cofactor Comment Organism Structure
FAD binding structure, overview Thermus caldophilus
NADP+
-
Thermus caldophilus
NADPH
-
Thermus caldophilus