Literature summary for 1.3.1.93 extracted from

Lehka, B.J.; Eichenberger, M.; Bjoern-Yoshimoto, W.E.; Vanegas, K.G.; Buijs, N.; Jensen, N.B.; Dyekjaer, J.D.; Jenssen, H.; Simon, E.; Naesby, M.
Improving heterologous production of phenylpropanoids in Saccharomyces cerevisiae by tackling an unwanted side reaction of Tsc13, an endogenous double-bond reductase (2017), FEMS Yeast Res., 17, fox004 .

Search for more literature for 1.3.1.93

Cloned(Commentary)

Cloned (Comment)	Organism
gene TSC13, TSC13 SSM library screening	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	with the aim of commercially interesting production of phenylpropanoids, such as flavonoids and stilbenoids, site saturation mutagenesis is used for modification of the enzyme to reduce the side activity without disrupting the natural function, identification of a number of amino acid changes which slightly increase flavonoid production but without reducing the formation of side product. The complementation of TSC13 by the gene homologue from plants essentially eliminates the unwanted side reaction, while retaining the productivity of phenylpropanoids in a simulated fed batch fermentation. The native open reading frame (ORF) of TSC13 is replaced by gene homologues from Arabidospis thaliana (AtECR), Gossypium hirsutum (GhECR2) and Malus domestica (MdECR), using a split URA3 cassette under the control of the native TSC13 promoter	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Synonyms

Synonyms	Comment	Organism
enoyl reductase	-	Saccharomyces cerevisiae
TSC13	-	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
additional information	homology modeling of Tsc13 based on the structure of a trans-2-enoyl reductase from Homo sapiens, PDB ID 1YXM	Saccharomyces cerevisiae
physiological function	the enoyl reductase Tsc13 is responsible for the accumulation of phloretic acid via reduction of p-coumaroyl-CoA. Tsc13 is an essential enzyme involved in fatty acid synthesis and cannot be deleted	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)