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Literature summary for 1.3.1.9 extracted from

  • Yang, H.; He, J.; Hu, F.; Zheng, C.; Yu, Z.
    Detection of Escherichia coli enoyl-ACP reductase using biarsenical-tetracysteine motif (2010), Bioconjug. Chem., 21, 1341-1348.
    View publication on PubMed
Search for more literature for 1.3.1.9

Application

Application Comment Organism
analysis construction of tetracysteine-tagged enzyme variants carrying the tag at the N-terminus, C-terminus, or both N- and C-terminus. All the tetracysteine-tagged FabI enzymes have high enzyme activities while the enhanced green fluorescent protein-tagged FabI exhaustively loses the activity. The binding between 4',5'-bis(1,3,2-dithioarsolan-2-yl)fuorescein, i.e. FlAsH reagent and tetracysteine motif is stable against high pressure, high field strength, high temperature, and ultrasound. A capillary zone electrophoresis system equipped with a laser-induced fluorescence detector has a detection limit of 10-16 M for the labeled proteins Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of tetracysteine-tagged enzyme variants carrying the tag at the N-terminus, C-terminus, or both N- and C-terminus. All the tetracysteine-tagged FabI enzymes have high enzyme activities while the enhanced green fluorescent protein-tagged FabI exhaustively loses the activity. The binding between 4',5'-bis(1,3,2-dithioarsolan-2-yl)fuorescein, i.e. FlAsH reagent and tetracysteine motif is stable against high pressure, high field strength, high temperature, and ultrasound. A capillary zone electrophoresis system equipped with a laser-induced fluorescence detector has a detection limit of 10-16 M for the labeled proteins Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
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Synonyms

Synonyms Comment Organism
FabI
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 Escherichia coli