Crystallization (Comment) | Organism |
---|---|
in complex with NAD and isoniazid, hanging drop vapour diffusion method,in 50 mM HEPES, pH 7.2, sodium citrate buffer and 5-10% 2-methyl-2,4-pentanediol | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
I21V | isoniazid-resistant mutant, loss of van der Waals interaction between NADH and the CD1 atom present in the valine residue leads to decrease of stability in binding of NADH in the active site of the protein | Mycobacterium tuberculosis |
S94A | isoniazid-resistant mutant, alteration in the binding network involving a conserved water molecule and O9 atom of molecule of NADH leads to increase of the flexibility of the conserved water molecule and decrease of the affinity of NADH by protein | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isoniazid | - |
Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WGR1 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WGR1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Subunits | Comment | Organism |
---|---|---|
tetramer | x-ray crystallography | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
2-trans-enoyl-ACP (CoA) reductase | - |
Mycobacterium tuberculosis |
InhA | - |
Mycobacterium tuberculosis |
NADH-dependent enoyl-acyl carrier protein reductase | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mycobacterium tuberculosis |