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Literature summary for 1.3.1.9 extracted from

  • Sivaraman, S.; Zwahlen, J.; Bell, A.F.; Hedstrom, L.; Tonge, P.J.
    Structure-activity studies of the inhibition of FabI, the enoyl reductase from Escherichia coli, by triclosan: kinetic analysis of mutant FabIs (2003), Biochemistry, 42, 4406-4413.
    View publication on PubMed
Search for more literature for 1.3.1.9

Protein Variants

Protein Variants Comment Organism
A197M no effect on triclosan inhibition Escherichia coli
F203L mutation decreases affinity of triclosan Escherichia coli
G93V mutation decreases affinity of triclosan Escherichia coli
M159T mutation decreases affinity of triclosan Escherichia coli
Y156F mutation decreases affinity of triclosan Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2,4,4'-trichloro-2'-hydroxydiphenyl ether trivial name triclosan Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, G93V mutant Escherichia coli
0.009
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, A197M mutant Escherichia coli
0.01
-
 NADH 25°C, pH 8.0, G93V mutant Escherichia coli
0.02
-
 NADH 25°C, pH 8.0 Escherichia coli
0.029
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, Y156F mutant Escherichia coli
0.03
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0 Escherichia coli
0.035
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, F203L mutant Escherichia coli
0.037
-
 NADH 25°C, pH 8.0, F203L mutant Escherichia coli
0.051
-
 NADH 25°C, pH 8.0, Y156F mutant Escherichia coli
0.079
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, M159T mutant Escherichia coli
0.35
-
 NADH 25°C, pH 8.0, A197M mutant Escherichia coli
0.4
-
 NADH 25°C, pH 8.0, M159T mutant Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AEK4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-2-dodecenoyl-CoA + NADH
-
 Escherichia coli dodecanoyl-CoA + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
FabI
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, M159T mutant Escherichia coli
1.75
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, Y156F mutant Escherichia coli
5
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, G93V mutant Escherichia coli
8.15
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, A197M mutant Escherichia coli
10.6
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, F203L mutant Escherichia coli
11.9
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Escherichia coli