Protein Variants | Comment | Organism |
---|---|---|
A182G | inactive | Streptomyces sp. |
A182L | the mutant with increased activity selectively catalyzes carboxylation over the regular reduction reaction | Streptomyces sp. |
V350A | the mutant shows increased activity compared to the wild type enzyme | Streptomyces sp. |
V350F | the mutant shows increased activity compared to the wild type enzyme | Streptomyces sp. |
V350G | the mutant shows increased activity compared to the wild type enzyme | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.089 | - |
cinnamoyl-CoA | mutant enzyme V350G, pH and temperature not specified in the publication | Streptomyces sp. | |
0.14 | - |
4-coumaroyl-CoA | mutant enzyme V350A, pH and temperature not specified in the publication | Streptomyces sp. | |
0.2 | - |
4-coumaroyl-CoA | mutant enzyme V350G, pH and temperature not specified in the publication | Streptomyces sp. | |
0.38 | - |
cinnamoyl-CoA | wild type enzyme, pH and temperature not specified in the publication | Streptomyces sp. | |
0.49 | - |
crotonyl-CoA | mutant enzyme V350F, pH and temperature not specified in the publication | Streptomyces sp. | |
1.17 | - |
crotonyl-CoA | mutant enzyme V350G, pH and temperature not specified in the publication | Streptomyces sp. | |
1.21 | - |
crotonyl-CoA | mutant enzyme V350A, pH and temperature not specified in the publication | Streptomyces sp. | |
1.31 | - |
crotonyl-CoA | mutant enzyme A182L, pH and temperature not specified in the publication | Streptomyces sp. | |
1.58 | - |
crotonyl-CoA | wild type enzyme, pH and temperature not specified in the publication | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | M1SQA1 | - |
- |
Streptomyces sp. NRRL 2288 | M1SQA1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-indolylacryloyl-CoA + NADPH + CO2 + H+ | - |
Streptomyces sp. | indolylmethylmalonyl-CoA + NADP+ | - |
? | |
3-indolylacryloyl-CoA + NADPH + CO2 + H+ | - |
Streptomyces sp. NRRL 2288 | indolylmethylmalonyl-CoA + NADP+ | - |
? | |
4-coumaroyl-CoA + NADPH + CO2 + H+ | - |
Streptomyces sp. | (2S)2-carboxy-3-(4-hydroxyphenyl)propionyl-CoA + NADP+ | - |
? | |
4-coumaroyl-CoA + NADPH + CO2 + H+ | - |
Streptomyces sp. NRRL 2288 | (2S)2-carboxy-3-(4-hydroxyphenyl)propionyl-CoA + NADP+ | - |
? | |
cinnamoyl-CoA + NADPH + CO2 + H+ | - |
Streptomyces sp. | (2S)2-carboxy-3-phenylpropionyl-CoA + NADP+ | - |
? | |
cinnamoyl-CoA + NADPH + CO2 + H+ | - |
Streptomyces sp. NRRL 2288 | (2S)2-carboxy-3-phenylpropionyl-CoA + NADP+ | - |
? | |
crotonyl-CoA + NADPH + CO2 + H+ | - |
Streptomyces sp. | (2S)-ethylmalonyl-CoA + NADP+ | - |
? | |
crotonyl-CoA + NADPH + CO2 + H+ | - |
Streptomyces sp. NRRL 2288 | (2S)-ethylmalonyl-CoA + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AntE | - |
Streptomyces sp. |
CCR | - |
Streptomyces sp. |
crotonyl-CoA carboxylase/reductase | - |
Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0675 | - |
crotonyl-CoA | mutant enzyme V350G, pH and temperature not specified in the publication | Streptomyces sp. | |
0.0688 | - |
cinnamoyl-CoA | wild type enzyme, pH and temperature not specified in the publication | Streptomyces sp. | |
0.0735 | - |
4-coumaroyl-CoA | mutant enzyme V350A, pH and temperature not specified in the publication | Streptomyces sp. | |
0.0877 | - |
cinnamoyl-CoA | mutant enzyme V350G, pH and temperature not specified in the publication | Streptomyces sp. | |
0.109 | - |
4-coumaroyl-CoA | mutant enzyme V350G, pH and temperature not specified in the publication | Streptomyces sp. | |
0.1197 | - |
crotonyl-CoA | mutant enzyme V350A, pH and temperature not specified in the publication | Streptomyces sp. | |
0.1263 | - |
crotonyl-CoA | mutant enzyme V350F, pH and temperature not specified in the publication | Streptomyces sp. | |
0.1519 | - |
crotonyl-CoA | mutant enzyme A182L, pH and temperature not specified in the publication | Streptomyces sp. | |
0.1755 | - |
crotonyl-CoA | wild type enzyme, pH and temperature not specified in the publication | Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Streptomyces sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0583 | - |
crotonyl-CoA | mutant enzyme V350G, pH and temperature not specified in the publication | Streptomyces sp. | |
0.111 | - |
crotonyl-CoA | wild type enzyme, pH and temperature not specified in the publication | Streptomyces sp. | |
0.115 | - |
crotonyl-CoA | mutant enzyme A182L, pH and temperature not specified in the publication | Streptomyces sp. | |
0.18 | - |
cinnamoyl-CoA | wild type enzyme, pH and temperature not specified in the publication | Streptomyces sp. | |
0.257 | - |
crotonyl-CoA | mutant enzyme V350A, pH and temperature not specified in the publication | Streptomyces sp. | |
0.257 | - |
crotonyl-CoA | mutant enzyme V350F, pH and temperature not specified in the publication | Streptomyces sp. | |
0.525 | - |
4-coumaroyl-CoA | mutant enzyme V350A, pH and temperature not specified in the publication | Streptomyces sp. | |
0.545 | - |
4-coumaroyl-CoA | mutant enzyme V350G, pH and temperature not specified in the publication | Streptomyces sp. | |
0.985 | - |
cinnamoyl-CoA | mutant enzyme V350G, pH and temperature not specified in the publication | Streptomyces sp. |