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Literature summary for 1.3.1.84 extracted from
- 3-Hydroxypropionyl-coenzyme A dehydratase and acryloyl-coenzyme A reductase, enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in the Sulfolobales (2009), J. Bacteriol., 191, 4572-4581.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: EDTA, Zn2+, other divalent cations | Sulfurisphaera tokodaii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
acryloyl-CoA | pH 6.0, 65°C | Sulfurisphaera tokodaii |  |
| 0.036 | - |
NADPH | pH 6.0, 65°C | Sulfurisphaera tokodaii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Sulfurisphaera tokodaii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | presence of 0.8 mol Zn2+ per mol of monomer, addition of Zn2+ is not stimulatory | Sulfurisphaera tokodaii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38600 | - |
1 * 39000, SDS-PAGE, 1 * 38600, calculated | Sulfurisphaera tokodaii |
| 39000 | - |
1 * 39000, SDS-PAGE, 1 * 38600, calculated | Sulfurisphaera tokodaii |
| 40000 | - |
x * 40000, SDS-PAGE | Metallosphaera sedula |
| 43000 | - |
gel filtration | Sulfurisphaera tokodaii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Metallosphaera sedula | - |
- |
- |
| Sulfurisphaera tokodaii | Q975C8 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.9 | - |
65°C | Metallosphaera sedula |
| 18.7 | - |
pH 6.0, 65°C | Sulfurisphaera tokodaii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 30% glycerol, stable for months | Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acryloyl-CoA + NADPH + H+ | - |
Sulfurisphaera tokodaii | propanoyl-CoA + NADP+ | - |
? | |
| acrylyl-CoA + NADPH + H+ | - |
Metallosphaera sedula | propanoyl-CoA + NADP+ | - |
? | |
| additional information | no substrate: NADH, crotonyl-CoA | Sulfurisphaera tokodaii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Metallosphaera sedula |
| monomer | 1 * 39000, SDS-PAGE, 1 * 38600, calculated | Sulfurisphaera tokodaii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acryloyl-coenzyme A reductase | - |
Metallosphaera sedula |
| acryloyl-coenzyme A reductase | - |
Sulfurisphaera tokodaii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13 | - |
NADPH | pH 6.0, 65°C | Sulfurisphaera tokodaii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Sulfurisphaera tokodaii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
half-maximal activity | Sulfurisphaera tokodaii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | no cofactor: NADH | Sulfurisphaera tokodaii | |
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Release 2023.1 (January 2023)
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