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Literature summary for 1.3.1.74 extracted from

  • Zhang, Y.; Teoh, K.; Reed, D.; Covello, P.
    Molecular cloning and characterization of Dbr1, a 2-alkenal reductase from Artemisia annua (2009), Botany, 87, 643-649.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Artemisia annua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0064
-
2E-nonenal pH 7.0, 30°C Artemisia annua
0.071
-
artemisinic aldehyde pH 7.0, 30°C Artemisia annua

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
x * 38500, calculated Artemisia annua

Organism

Organism UniProt Comment Textmining
Artemisia annua
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information Dbr1 expression is readily detectable in aerial parts of the plant, but not in the roots Artemisia annua
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-4-hydroxynon-2-enal + NADPH + H+
-
Artemisia annua 4-hydroxynonanal + NADP+
-
?
2E-hexenal + NADPH + H+ at 12% of the rate with 2E-nonenal Artemisia annua hexanal + NADP+
-
?
2E-nonenal + NADPH + H+
-
Artemisia annua nonanal + NADP+
-
?
artemisinic aldehyde + NADPH + H+ at 1.9% of the rate with 2E-nonenal Artemisia annua dihydroartemisinic aldehyde + NADP+ 88% 11S- and 12% 11R-isomer ?

Subunits

Subunits Comment Organism
? x * 38500, calculated Artemisia annua

Synonyms

Synonyms Comment Organism
Dbr1
-
Artemisia annua
double bond reductase
-
Artemisia annua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Artemisia annua

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADH Artemisia annua
NADPH
-
Artemisia annua