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Literature summary for 1.3.1.72 extracted from

  • Rodriguez-Acebes, S.; de la Cueva, P.; Fernandez-Hernando, C.; Ferruelo, A.J.; Lasuncion, M.A.; Rawson, R.B.; Martinez-Botas, J.; Gomez-Coronado, D.
    Desmosterol can replace cholesterol in sustaining cell proliferation and regulating the SREBP pathway in a sterol-DELTA24-reductase-deficient cell line (2009), Biochem. J., 420, 305-315.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
quantitative real-time PCR DHCR24 expression analysis Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5alpha-cholest-7-en-3beta-ol + NADP+ Mus musculus
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 5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information no activity in J774 cells, that lack a full-length transcript for DHCR24. J774 cells are able to grow indefinitely after the virtually total replacement of cholesterol by desmosterol, i.e. J774-D cells Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-cholest-7-en-3beta-ol + NADP+
-
 Mus musculus 5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
3beta -hydroxysterol DELTA24-reductase
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 Mus musculus
DHCR24
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 Mus musculus
sterol-DELTA24-reductase
-
 Mus musculus

General Information

General Information Comment Organism
metabolism desmosterol suffice as a cholesterol replacement. Although a wide range of sterols and cholesterol analogues such as beta-sitosterol, ergosterol and 5beta-cholestan-3beta-ol are unable to support cell proliferation, while 5alpha-cholestan-3beta-ol, i.e. dihydrocholesterol, can do so, pathway regulation, detailed overview Mus musculus
additional information J774 cells, that lack a full-length transcript for DHCR24, consequently accumulate desmosterol Mus musculus
additional information while cholesterol itself may not be unique in its ability to sustain mammalian life, sterols very much like cholesterol, such as desmosterol, appear to be absolutely required Mus musculus