Application | Comment | Organism |
---|---|---|
agriculture | inhibition of phytosterol metabolism to cholesterol by interfering with DELTA24-sterol reductase activity presents a unique target site that might be exploited further as a selective, biorational insect-control technology | Manduca sexta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
24(R,S),25-epimino-lanosterol | iminiolanosterol, IL, potent inhibitor, IC50: 0.002 mM | Manduca sexta | |
25-Azacholesterol | - |
Manduca sexta | |
azasteroids | mechanism of inhibition | Manduca sexta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Manduca sexta | |
0.0026 | - |
5alpha-cholesta-5,24-dien-3beta-ol | desmosterol | Manduca sexta | |
0.018 | - |
4,4',14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | lanosterol | Manduca sexta | |
0.033 | - |
Cycloartenol | - |
Manduca sexta |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | microsome-bound | Manduca sexta | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
desmosterol + NADPH | Manduca sexta | 5alpha-cholesta-5,24-dien-3beta-ol | cholesterol + NADP+ | - |
? | |
additional information | Manduca sexta | enzyme of phytosterol metabolism to cholesterol | ? | - |
? | |
additional information | Manduca sexta | reduction of 24,25-double bond | ? | - |
? | |
additional information | Manduca sexta | insects are unable to synthesize sterols de novo and acquire sterols from their diet or from symbionts, sterol 24,25-reductase is an important enzyme of the pathway for the transformation of sitosterol, 24alpha-ethylcholesterol, together with stigmasterol, 24alpha-ethylcholesta-5,22-dien-3beta-ol, the predominant phytosterol in tobacco, to cholesterol | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
silkworm | - |
Manduca sexta | - |
tobacco hornworm | - |
Purification (Comment) | Organism |
---|---|
- |
Manduca sexta |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5alpha-cholest-7-en-3beta-ol + NADP+ = 5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+ | mechanism | Manduca sexta | |
5alpha-cholest-7-en-3beta-ol + NADP+ = 5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+ | stereochemistry | Bombyx mori | |
5alpha-cholest-7-en-3beta-ol + NADP+ = 5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+ | stereochemistry | Manduca sexta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Manduca sexta | - |
midgut | - |
Manduca sexta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Manduca sexta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-cholesta-7,24-dien-3beta-ol + NADPH | - |
Manduca sexta | 5alpha-cholesta-7-en-3beta-ol + NADP+ | lathosterol | ? | |
cycloartenol + NADPH + H+ | - |
Manduca sexta | cycloartanol + NADP+ | - |
? | |
desmosterol + NADPH | preferred, best substrate | Manduca sexta | cholesterol + NADP+ | - |
? | |
desmosterol + NADPH | 5alpha-cholesta-5,24-dien-3beta-ol | Bombyx mori | cholesterol + NADP+ | - |
? | |
desmosterol + NADPH | 5alpha-cholesta-5,24-dien-3beta-ol | Manduca sexta | cholesterol + NADP+ | - |
? | |
lanosterol + NADPH | 4,4',14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Manduca sexta | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
additional information | substrate specificity | Manduca sexta | ? | - |
? | |
additional information | sterol 24,25-double bond reduction | Bombyx mori | ? | - |
? | |
additional information | sterol 24,25-double bond reduction | Manduca sexta | ? | - |
? | |
additional information | substrate specificity studies | Manduca sexta | ? | - |
? | |
additional information | enzyme of phytosterol metabolism to cholesterol | Manduca sexta | ? | - |
? | |
additional information | reduction of 24,25-double bond | Manduca sexta | ? | - |
? | |
additional information | insects are unable to synthesize sterols de novo and acquire sterols from their diet or from symbionts, sterol 24,25-reductase is an important enzyme of the pathway for the transformation of sitosterol, 24alpha-ethylcholesterol, together with stigmasterol, 24alpha-ethylcholesta-5,22-dien-3beta-ol, the predominant phytosterol in tobacco, to cholesterol | Manduca sexta | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Manduca sexta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bombyx mori | |
NADPH | NADPH-linked | Manduca sexta |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.002 | - |
iminiolanosterol, IL, potent inhibitor, IC50: 0.002 mM | Manduca sexta | 24(R,S),25-epimino-lanosterol |