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Literature summary for 1.3.1.70 extracted from

  • Prakash, A.; Kasbekar, D.P.
    Genes encoding chimeras of Neurospora crassa erg-3 and human TM7SF2 proteins fail to complement Neurospora and yeast sterol C-14 reductase mutants (2002), J. Biosci., 27, 105-112.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
a Neurospora crassa enzyme-deficient erg-3 mutant strain mat a and a yeast erg-4 mutant strain AP2 cannot be complemented by expression of 6 chimeric enzyme mutants variants constructed from the enzyme encoded by gene TM7SF2 and the Neurospora crassa gene erg-3 encoded enzyme Homo sapiens
an enzyme-deficient erg-3 mutant strain mat a and a yeast erg-4 mutant strain AP2 cannot be complemented by expression of 6 chimeric enzyme mutants variants constructed from the enzyme encoded by erg-3 and the human gene TM7SF2 encoding the SR-1 protein Neurospora crassa

Protein Variants

Protein Variants Comment Organism
E475Q site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
G225C site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
Y317F site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme plays a key role in sterol biosynthesis, sterol content in vivo ?
-
?
additional information Neurospora crassa enzyme plays a key role in sterol biosynthesis, sterol content in vivo ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
gene TM7SF2, enzyme SR-1
-
Neurospora crassa
-
gene erg-3
-

Reaction

Reaction Comment Organism Reaction ID
4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H+ residues G225, Y317, and E475 are not essential for activity Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme plays a key role in sterol biosynthesis, sterol content in vivo Homo sapiens ?
-
?
additional information enzyme plays a key role in sterol biosynthesis, sterol content in vivo Neurospora crassa ?
-
?

Synonyms

Synonyms Comment Organism
SR-1
-
Homo sapiens
sterol C-14 reductase
-
Homo sapiens
sterol C-14 reductase
-
Neurospora crassa