Cloned (Comment) | Organism |
---|---|
cDNA expression in COS-7 cells | Bos taurus |
RT-PCR cloning | Bos taurus |
sterol DELTA14-reductase mRNA expression in bovine tisssues, high levels in liver and brain | Bos taurus |
TM7SF2 gene corresponds to DELTA14-SR, sterol DELTA14-reductase | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | integral protein of endoplasmic reticulum | Homo sapiens | 5783 | - |
endoplasmic reticulum | integral protein of endoplasmic reticulum | Bos taurus | 5783 | - |
membrane | transmembrane domains | Bos taurus | 16020 | - |
microsome | - |
Homo sapiens | - |
- |
microsome | - |
Bos taurus | - |
- |
additional information | subcellular localization | Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
SDS-PAGE | Bos taurus |
46750 | - |
cloned cDNA, calculated from amino-acid sequence. The discrepancy between the apparent molecular mass of 38000 Da estimated by SDS-PAGE and the calculated molecular mass may be due to an aberrant electrophoretic migration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway | ? | - |
? | |
additional information | Bos taurus | 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway | ? | - |
? | |
additional information | Homo sapiens | important essential enzyme of cholesterol biosynthesis from lanosterol | ? | - |
? | |
additional information | Bos taurus | important essential enzyme of cholesterol biosynthesis from lanosterol | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
bovine | - |
Homo sapiens | - |
human | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH | - |
Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ | - |
? | |
4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH | C29DELTA8,14,24 | Bos taurus | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ | - |
? | |
4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + NADPH | - |
Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + NADPH | - |
Bos taurus | 4,4-dimethyl-5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
5alpha-cholesta-8,14-dien-3beta-ol + NADPH | DELTA8,14-cholestadien-3beta-ol | Bos taurus | 5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
5alpha-cholesta-8,14-dien-3beta-ol + NADPH | C27DELTA8,14 | Bos taurus | 5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
additional information | enzyme acts on a range of steroids with a 14(15)-double bond | Homo sapiens | ? | - |
? | |
additional information | enzyme acts on a range of steroids with a 14(15)-double bond | Bos taurus | ? | - |
? | |
additional information | 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway | Homo sapiens | ? | - |
? | |
additional information | 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway | Bos taurus | ? | - |
? | |
additional information | important essential enzyme of cholesterol biosynthesis from lanosterol | Homo sapiens | ? | - |
? | |
additional information | important essential enzyme of cholesterol biosynthesis from lanosterol | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens | |
NADPH | - |
Bos taurus |