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Literature summary for 1.3.1.70 extracted from
- Cloning and expression of sterol DELTA14-reductase from bovine liver (2002), Eur. J. Biochem., 269, 283-290.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA expression in COS-7 cells | Bos taurus |
| RT-PCR cloning | Bos taurus |
| sterol DELTA14-reductase mRNA expression in bovine tisssues, high levels in liver and brain | Bos taurus |
| TM7SF2 gene corresponds to DELTA14-SR, sterol DELTA14-reductase | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | integral protein of endoplasmic reticulum | Homo sapiens | 5783 | - |
| endoplasmic reticulum | integral protein of endoplasmic reticulum | Bos taurus | 5783 | - |
| membrane | transmembrane domains | Bos taurus | 16020 | - |
| microsome | - |
Homo sapiens | - |
- |
| microsome | - |
Bos taurus | - |
- |
| additional information | subcellular localization | Bos taurus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
SDS-PAGE | Bos taurus |
| 46750 | - |
cloned cDNA, calculated from amino-acid sequence. The discrepancy between the apparent molecular mass of 38000 Da estimated by SDS-PAGE and the calculated molecular mass may be due to an aberrant electrophoretic migration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway | ? | - |
? |  |
| additional information | Bos taurus | 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway | ? | - |
? | |
| additional information | Homo sapiens | important essential enzyme of cholesterol biosynthesis from lanosterol | ? | - |
? | |
| additional information | Bos taurus | important essential enzyme of cholesterol biosynthesis from lanosterol | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
bovine | - |
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
| liver | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH | - |
Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ | - |
? | |
| 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH | C29DELTA8,14,24 | Bos taurus | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ | - |
? | |
| 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + NADPH | - |
Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
| 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + NADPH | - |
Bos taurus | 4,4-dimethyl-5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
| 5alpha-cholesta-8,14-dien-3beta-ol + NADPH | DELTA8,14-cholestadien-3beta-ol | Bos taurus | 5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
| 5alpha-cholesta-8,14-dien-3beta-ol + NADPH | C27DELTA8,14 | Bos taurus | 5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
| additional information | enzyme acts on a range of steroids with a 14(15)-double bond | Homo sapiens | ? | - |
? | |
| additional information | enzyme acts on a range of steroids with a 14(15)-double bond | Bos taurus | ? | - |
? | |
| additional information | 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway | Homo sapiens | ? | - |
? | |
| additional information | 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway | Bos taurus | ? | - |
? | |
| additional information | important essential enzyme of cholesterol biosynthesis from lanosterol | Homo sapiens | ? | - |
? | |
| additional information | important essential enzyme of cholesterol biosynthesis from lanosterol | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Homo sapiens | |
| NADPH | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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