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Literature summary for 1.3.1.70 extracted from
- DELTA14-Sterol reductase in Saccharomyces cerevisiae (1978), Biochim. Biophys. Acta, 531, 301-307.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Chelating agents | slight activation | Saccharomyces cerevisiae |  |
| EDTA | slight activation | Saccharomyces cerevisiae | |
General Stability
| General Stability | Organism |
|---|---|
| enzyme very labile | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 15-azasterol | 15-aza-24-methylene-D-homocholestadiene; potent antimycotic agent, designated A258223, non-competitively inhibition at very low concentrations, Ki: 0.000002 mM | Saccharomyces cerevisiae | |
| Detergents | enzyme solubilization by detergents: inactivation | Saccharomyces cerevisiae | |
| Mg2+ | - |
Saccharomyces cerevisiae | |
| additional information | no inhibition by ergosterol, up to 0.2 mM | Saccharomyces cerevisiae | |
| Triton X-100 | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Saccharomyces cerevisiae | |
| additional information | - |
additional information | Km for 4,4-dimethylcholesta-8,14,24-trienol, probably the natural substrate of DELTA14-reductase in yeast, may be different because of interactions of enzyme with C-4 methyl groups | Saccharomyces cerevisiae | |
| 0.06 | - |
ergosta-8,14-dien-3beta-ol | ignosterol, Lineweaver-Burk plots | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | cytoplasmic fraction | Saccharomyces cerevisiae | 5737 | - |
| additional information | distribution | Saccharomyces cerevisiae | - |
- |
| additional information | enzyme non-mitochondrial, greatest activity in hard protein pellet, suggesting that enzyme is an insoluble protein | Saccharomyces cerevisiae | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH | Saccharomyces cerevisiae | probably the natural substrate | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ | - |
? | |
| 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH | Saccharomyces cerevisiae 3701B | probably the natural substrate | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ | - |
? | |
| additional information | Saccharomyces cerevisiae | reduction of DELTA8,14-intermediate in ergosterol biosynthetic pathway to DELTA8-intermediate occurs by the trans addition of two hydrogens to the 14alpha- and 15beta-positions | ? | - |
? | |
| additional information | Saccharomyces cerevisiae | enzyme of biosynthetic pathways of ergosterol, major sterol in yeast, and cholesterol including a DELTA8,14-sterol intermediate as result of demethylation of lanosterol at C-14 | ? | - |
? | |
| additional information | Saccharomyces cerevisiae 3701B | reduction of DELTA8,14-intermediate in ergosterol biosynthetic pathway to DELTA8-intermediate occurs by the trans addition of two hydrogens to the 14alpha- and 15beta-positions | ? | - |
? | |
| additional information | Saccharomyces cerevisiae 3701B | enzyme of biosynthetic pathways of ergosterol, major sterol in yeast, and cholesterol including a DELTA8,14-sterol intermediate as result of demethylation of lanosterol at C-14 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
3701B | - |
| Saccharomyces cerevisiae 3701B | - |
3701B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
| Sephadex columns to de-salt enzyme preparation: 5fold activation | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH | - |
Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ | - |
? | |
| 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH | probably the natural substrate | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ | - |
? | |
| 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH | - |
Saccharomyces cerevisiae 3701B | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ | - |
? | |
| 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH | probably the natural substrate | Saccharomyces cerevisiae 3701B | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ | - |
? | |
| ergosta-8,14-dien-3beta-ol + NADPH | DELTA8,14-sterol, ignosterol | Saccharomyces cerevisiae | ergosta-8-en-3beta-ol + NADP+ | - |
? | |
| additional information | enzyme acts on a range of steroids with a 14(15)-double bond | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | reduction of DELTA8,14-intermediate in ergosterol biosynthetic pathway to DELTA8-intermediate occurs by the trans addition of two hydrogens to the 14alpha- and 15beta-positions | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | enzyme of biosynthetic pathways of ergosterol, major sterol in yeast, and cholesterol including a DELTA8,14-sterol intermediate as result of demethylation of lanosterol at C-14 | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | enzyme acts on a range of steroids with a 14(15)-double bond | Saccharomyces cerevisiae 3701B | ? | - |
? | |
| additional information | reduction of DELTA8,14-intermediate in ergosterol biosynthetic pathway to DELTA8-intermediate occurs by the trans addition of two hydrogens to the 14alpha- and 15beta-positions | Saccharomyces cerevisiae 3701B | ? | - |
? | |
| additional information | enzyme of biosynthetic pathways of ergosterol, major sterol in yeast, and cholesterol including a DELTA8,14-sterol intermediate as result of demethylation of lanosterol at C-14 | Saccharomyces cerevisiae 3701B | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 6.8 | about 50% of maximum activity | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | best stimulation | Saccharomyces cerevisiae | |
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