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Literature summary for 1.3.1.67 extracted from

  • Whited, M.G.; McCombie, W.R.
    Identification of cis-diols as intermediates in the oxidation of aromatic acids by a strain of Pseudomonas putida that contains a TOL plasmid (1986), J. Bacteriol., 166, 1028-1039.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.67

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carboxylic acid diol + NAD+ Pseudomonas putida
-
 catechol + NADH
-
 ?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+ Pseudomonas putida no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate 4-methylcatechol + NADH + H+ + CO2
-
 ?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+ Pseudomonas putida BG1 no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate 4-methylcatechol + NADH + H+ + CO2
-
 ?
cis-p-toluate diol + NAD+ Pseudomonas putida metabolism of toluene 4-methylcatechol + NADH + H+ + CO2
-
 ?
cis-p-toluate diol + NAD+ Pseudomonas putida BG1 metabolism of toluene 4-methylcatechol + NADH + H+ + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 harboring TOL plasmid
-
Pseudomonas putida BG1
-
 harboring TOL plasmid
-

Purification (Commentary)

Purification (Comment) Organism
French press, DEAE-cellulose column, dialysis, Red A column, ultrafiltration, purified 38fold Pseudomonas putida

Reaction

Reaction Comment Organism Reaction ID
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD(P)+ = 4-methylcatechol + NAD(P)H + H+ + CO2 enzyme utilizes NAD+ but not NAD(P) Pseudomonas putida
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.067
-
 with plasmid pDTG501 and carbon source p-toluate Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dihydroxycyclohexa-3,5-diene 1-carboxylate + NAD+
-
 Pseudomonas putida catechol + NADH
-
 ?
1,2-dihydroxycyclohexa-3,5-diene 1-carboxylate + NAD+
-
 Pseudomonas putida BG1 catechol + NADH
-
 ?
carboxylic acid diol + NAD+
-
 Pseudomonas putida catechol + NADH
-
 ?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
-
 Pseudomonas putida 4-methylcatechol + NADH + H+ + CO2
-
 ?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+ no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate Pseudomonas putida 4-methylcatechol + NADH + H+ + CO2
-
 ?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
-
 Pseudomonas putida BG1 4-methylcatechol + NADH + H+ + CO2
-
 ?
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+ no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate Pseudomonas putida BG1 4-methylcatechol + NADH + H+ + CO2
-
 ?
cis-p-toluate diol + NAD+
-
 Pseudomonas putida 4-methylcatechol + NADH + H+ + CO2
-
 ?
cis-p-toluate diol + NAD+ metabolism of toluene Pseudomonas putida 4-methylcatechol + NADH + H+ + CO2
-
 ?
cis-p-toluate diol + NAD+
-
 Pseudomonas putida BG1 4-methylcatechol + NADH + H+ + CO2
-
 ?
cis-p-toluate diol + NAD+ metabolism of toluene Pseudomonas putida BG1 4-methylcatechol + NADH + H+ + CO2
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pseudomonas putida