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Literature summary for 1.3.1.6 extracted from
- A sodium-stimulated membrane-bound fumarate reductase system in Bacteroides amylophilus (1985), Arch. Microbiol., 143, 157-162.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | up to 17fold increase in methyl viologen dependent activity, loss of NADH dependent activity | Ruminobacter amylophilus |  |
General Stability
| General Stability | Organism |
|---|---|
| inactivation by irradiation at 254 nm | Ruminobacter amylophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-n-heptyl-4-hydroxyquinoline-N-oxide | strong inhibitor | Ruminobacter amylophilus | |
| Acriflavin | specific inhibition of NADH dependent activity | Ruminobacter amylophilus | |
| ClHgSO3H | inhibition with all cofactors except the methyl viologen dependent activity | Ruminobacter amylophilus | |
| KCN | inhibition with all cofactors except the methyl viologen dependent activity | Ruminobacter amylophilus | |
| additional information | inactivation by irradiation at 254 nm, can be partially restored by addition of 2,3-dimethyl-1,4-naphthoquinone | Ruminobacter amylophilus | |
| Triton X-100 | complete loss of NADH dependent activity at concentration of 0.05%, up to 17fold increase in methyl viologen dependent activity | Ruminobacter amylophilus | |
| Zn2+ | strong inhibitor | Ruminobacter amylophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
NADH | - |
Ruminobacter amylophilus | |
| 0.025 | - |
fumarate | - |
Ruminobacter amylophilus | |
| 0.086 | - |
FMNH2 | - |
Ruminobacter amylophilus | |
| 0.28 | - |
FADH2 | - |
Ruminobacter amylophilus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Ruminobacter amylophilus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | slight activation | Ruminobacter amylophilus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumarate + NADH + H+ | Ruminobacter amylophilus | - |
succinate + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruminobacter amylophilus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| sensitive to air oxidation, loss of activity | Ruminobacter amylophilus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.14 | 0.25 | with NADH, FMNH2 and FADH2 | Ruminobacter amylophilus |
| 0.84 | - |
with benzyl- or mehtyl viologen | Ruminobacter amylophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumarate + FADH2 | - |
Ruminobacter amylophilus | succinate + FAD | - |
r | |
| fumarate + FMNH2 | - |
Ruminobacter amylophilus | succinate + FMN | - |
r | |
| fumarate + NADH + H+ | - |
Ruminobacter amylophilus | succinate + NAD+ | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FADH2 | - |
Ruminobacter amylophilus | |
| FMNH2 | - |
Ruminobacter amylophilus | |
| NADH | - |
Ruminobacter amylophilus | |
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