Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.51 extracted from

  • Fischer, D.; Ebenau-Jehle, C.; Grisebach, H.
    Phytoalexin synthesis in soybean: purification and characterization of NADPH: 2'-hydroxydaidzein oxidoreductase from elicitor-challenged soybean cell culture (1990), Arch. Biochem. Biophys., 276, 390-395.
    View publication on PubMed
Search for more literature for 1.3.1.51

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 2'-Hydroxydaidzein
-
 Glycine max
0.056
-
 NADPH
-
 Glycine max
0.06
-
 2'-Hydroxyformomonetin
-
 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34700
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Glycine max specific role in the biosynthesis of glyceollin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cell culture elicitor-induced Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-Hydroxydaidzein + NADH 8% of the reaction with NADP+ Glycine max 2'-Hydroxydihydrodaidzein + NAD+
-
 ?
2'-Hydroxydaidzein + NADPH
-
 Glycine max 2'-Hydroxydihydrodaidzein + NADP+
-
 ?
2'-Hydroxyformomonetin + NADPH
-
 Glycine max 2'-Hydroxydihydroformonetin + NADP+
-
 ?
2'-Hydroxygenistein + NADPH 10% of the reaction relative to 2'-hydroxydaidzein Glycine max 2'-Hydroxydihydrogenistein + NADP+
-
 ?
additional information no activity with: biochanin A, daidzein, genistein, apigenin, kaempferol, quercetin Glycine max ?
-
 ?
additional information specific role in the biosynthesis of glyceollin Glycine max ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34700, SDS-PAGE Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 pH 5.5: about 40% of maximal activity, pH 9.0: about 45% of maximal activity Glycine max