Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
15-ketoprostaglandin E1 | - |
Sus scrofa | |
0.0083 | - |
15-ketoprostaglandin E1 | recombinant enzyme | Sus scrofa | |
0.01 | - |
leukotriene B4 | recombinant enzyme | Sus scrofa | |
0.015 | - |
NADPH | - |
Sus scrofa | |
0.016 | - |
NADPH | recombinant enzyme | Sus scrofa | |
0.0182 | - |
15-ketoprostaglandin E2 | recombinant enzyme | Sus scrofa | |
0.019 | - |
15-ketoprostaglandin E2 | - |
Sus scrofa | |
0.088 | 0.094 | NADH | - |
Bos taurus | |
0.153 | - |
NADH | - |
Sus scrofa | |
0.16 | - |
NADH | recombinant enzyme | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and recombinant enzyme | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Sus scrofa | - |
lung | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.56 | - |
- |
Sus scrofa |
2.6 | - |
recombinant enzyme expressed in Escherichia coli | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5S,12R)-dihydroxy-(6E,8Z,10Z,14E)-eicosatetraenoic acid + NADH | trivial name leukotriene B4, enzyme exhibits a 15-ketoprostaglandin-13-reductase and a very low leukotriene B4 12-hydroxydehydrogenase activity | Sus scrofa | ? | - |
? | |
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H | - |
Sus scrofa | (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ | - |
ir | |
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H | - |
Bos taurus | (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ | - |
ir | |
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H | - |
Sus scrofa | 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+ | - |
? | |
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H | - |
Bos taurus | 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+ | - |
? | |
15-ketoprostaglandin E1 + NADPH | - |
Sus scrofa | ? | - |
? | |
15-ketoprostaglandin E2 + NADH + H+ | - |
Bos taurus | 13,14-dihydro-15-ketoprostaglandin E2 + NAD+ | - |
r | |
leukotriene B4 + NADPH | - |
Sus scrofa | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.517 | - |
15-ketoprostaglandin E1 | cofactor NADH | Sus scrofa | |
1.52 | - |
15-ketoprostaglandin E1 | cofactor NADPH | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Bos taurus | |
NAD(P)H | 3fold higher activity with NADPH compared to NADH | Sus scrofa |