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Literature summary for 1.3.1.46 extracted from

  • Schlieper, D.; Barz, W.
    NADPH:biochanin A oxidoreductase from the fungus Fusarium javanicum (1987), Phytochemistry, 26, 2495-2498.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
Biochanin A
-
Fusarium solani-melongenae
0.19
-
NADPH
-
Fusarium solani-melongenae

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Fusarium solani-melongenae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000 88000 gel filtration Fusarium solani-melongenae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+ Fusarium solani-melongenae trivial name biochanin A (3S)-2-dihydrobiochanin A + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Fusarium solani-melongenae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Fusarium solani-melongenae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.355
-
-
Fusarium solani-melongenae

Storage Stability

Storage Stability Organism
-25°C, 20% v/v glycerol, with NADPH, but unstable with NADP+ Fusarium solani-melongenae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,7,3'-trihydroxy-4'-methoxyisoflavone + NADPH + H+ trivial name pratensein, 78% of activity with biochanin A Fusarium solani-melongenae 2-dihydropratensein + NADP+
-
?
5,7,3'-trihydroxyisoflavone + NADPH + H+ trivial name genistein, 44% of activity with biochanin A Fusarium solani-melongenae 2-dihydrogenistein + NADP+
-
?
6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+ highly specific for 5,7-hydroxy group and some specificity for 4'-substituent of isoflavones, no reaction with flavones, chalcones, 5-deoxyisoflavones Fusarium solani-melongenae (3S)-2-dihydrobiochanin A + NADP+ hydrated at 2 position or ring A of isoflavone ?
6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+ trivial name biochanin A Fusarium solani-melongenae (3S)-2-dihydrobiochanin A + NADP+
-
?
6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+ trivial name biochanin A Fusarium solani-melongenae (3S)-2-dihydrobiochanin A + NADP+ hydrated at 2 position or ring A of isoflavone ?
biochanin A + NADPH + H+
-
Fusarium solani-melongenae dihydrobiochanin A + NADP+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 43000-44000, SDS-PAGE Fusarium solani-melongenae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
Fusarium solani-melongenae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
inactivation above Fusarium solani-melongenae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Fusarium solani-melongenae

pH Range

pH Minimum pH Maximum Comment Organism
5 8
-
Fusarium solani-melongenae

Cofactor

Cofactor Comment Organism Structure
NADPH 30% of maximum activity with NADH Fusarium solani-melongenae