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Literature summary for 1.3.1.45 extracted from

  • Cheng, Q.; Li, N.; Dong, L.; Zhang, D.; Fan, S.; Jiang, L.; Wang, X.; Xu, P.; Zhang, S.
    Overexpression of soybean isoflavone reductase (GmIFR) enhances resistance to phytophthora sojae in soybean (2015), Front. Plant Sci., 6, 1024 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.45

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, transient expression of GFP-tagged enzyme in Glycine max protoplasts' cytoplasm, recombinant overexpression of the enzyme under the control of a CaMV35S promoter via Agrobacterium tumefaciens strain LBA4404 and tri-parental mating in transgenic Glycine max plants, quantitative real-time PCR enzyme expression analysis, recombinant expression of Nterminally His6-tagged enzyme in Escherichia coli strain Transetta (DE3) Glycine max

Protein Variants

Protein Variants Comment Organism
additional information enzyme overexpression in Glycine max transgenic plants results in highly reduced daidzein levels, while the relative content of glyceollins in transgenic plants is significantly higher than that of non-transgenic plants. The relative expression levels of reactive oxygen species (ROS) of transgenic soybean plants are significantly lower than those of non-transgenic plants after incubation with Phytophthora sojae. Overexpression of GmIFR in soybean seed affects isoflavone and glyceollins expression levels, as well as the transcriptional level of multiple genes involved in the phenylpropanal pathway Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm subcellular localization analysis in transgenic protoplasts Glycine max 5737
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-hydroxyformononetin + NADPH + H+ Glycine max GmIFR is an isoflavone reductase vestitone + NADP+
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 ?

Organism

Organism UniProt Comment Textmining
Glycine max C6TER4 cv. Suinong10
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Source Tissue

Source Tissue Comment Organism Textmining
hypocotyl
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 Glycine max
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seedling
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 Glycine max
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-hydroxyformononetin + NADPH + H+
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 Glycine max vestitone + NADP+
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 ?
2'-hydroxyformononetin + NADPH + H+ GmIFR is an isoflavone reductase Glycine max vestitone + NADP+
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 ?

Subunits

Subunits Comment Organism
? x * 34920, sequence calculation, x * 34000, recombinant enzyme, SDS-PAGE Glycine max
More The predicted three-dimensional model prediction Glycine max

Synonyms

Synonyms Comment Organism
GmIFR
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 Glycine max
IFR
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 Glycine max
isoflavone reductase
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 Glycine max
LOC100786578 locus name Glycine max

Cofactor

Cofactor Comment Organism Structure
NADP+
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 Glycine max

pI Value

Organism Comment pI Value Maximum pI Value
Glycine max sequence calculation
-
 6.33

Expression

Organism Comment Expression
Glycine max the enzyme expression is significantly induced by infection with Phytophthora sojae, as well as by treatment with ethephon, abscisic acid, and salicylic acid up

General Information

General Information Comment Organism
evolution isoflavone reductases (IFRs) are unique to the plant kingdom. The enzyme is a member of the soybean isoflavone reductase gene family GmIF Glycine max
physiological function isoflavonereductase (IFR)is an enzyme involved in the biosynthetic pathway of isoflavonoid phytoalexin in plants. The enzyme is considered to have a crucial role in plant response to various biotic and abiotic environmental stresses. Overexpression of soybean isoflavone reductase (GmIFR) enhances resistance to Phytophthora sojae in soybean. Enzyme GmIFR might play an important role as an antioxidant to reduce ROS in soybean Glycine max