Literature summary for 1.3.1.44 extracted from

Poletto, S.S.; da Fonseca, I.O.; de Carvalho, L.P.; Basso, L.A.; Santos, D.S.
Selection of an Escherichia coli host that expresses mutant forms of Mycobacterium tuberculosis 2-trans enoyl-ACP(CoA) reductase and 3-ketoacyl-ACP(CoA) reductase enzymes (2004), Protein Expr. Purif., 34, 118-125.

Search for more literature for 1.3.1.44

Application

Application	Comment	Organism
medicine	enzyme is a target for drugs in treatment of tuberculosis	Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment)	Organism
gene inhA, overexpression of stable mutant enzymes in Escherichia coli strain BL21(DE3)	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
K165A	site-directed mutagenesis, mutation of putative catalytic residue	Mycobacterium tuberculosis
K165Q	site-directed mutagenesis, mutation of putative catalytic residue	Mycobacterium tuberculosis
additional information	construction of stable mutants for optimization of expression of enzyme in Escherichia coli, absolutely dependent on IPTG, phenotype alterations, overview	Mycobacterium tuberculosis
Y158F	site-directed mutagenesis, mutation of putative catalytic residue, mutant can only be stably expressed in Escherichia coli strain BL21(DE3) if altered to a soluble enzyme form dependent on IPTG induction	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
isoniazid	drug to treat tuberculosis	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	enzyme is a component of fatty acid synthase type II	-

Synonyms

Synonyms	Comment	Organism
ENR	-	Mycobacterium tuberculosis
More	enzyme belongs to the short-chain dehydrogenase/reductase family, enzyme is a component of fatty acid synthase type II	Mycobacterium tuberculosis
trans-2-enoyl-ACP(CoA) reductase	-	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
NADH	dependent on	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)