Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetic acid | 1 mM, 34% inhibition | Escherichia coli | |
p-hydroxymercuribenzoate | 1 mM, 77% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
cis-2-octenoyl-CoA | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0005 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, ammonium sulfate precipitate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-2-decenoyl-CoA + NADPH + H+ | no reduction of cis-3-enoyl substrates | Escherichia coli | decanoyl-CoA + NADP+ | - |
? | |
cis-2-dodecenoyl-CoA + NADPH + H+ | - |
Escherichia coli | dodecanoyl-CoA + NADP+ | - |
? | |
cis-2-octenoyl-CoA + NADPH + H+ | - |
Escherichia coli | octanoyl-CoA + NADP+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
no loss of activity after 30 min | Escherichia coli |
55 | - |
10 min, inactivation | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |