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Literature summary for 1.3.1.36 extracted from
- Partial purification and characterization of geissoschizzine dehydrogenase from suspension cultures of Catharanthus roseus (1982), Phytochemistry, 21, 1585-1588.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000077 | - |
NADP+ | - |
Catharanthus roseus |  |
| 0.083 | - |
Geissoschizine | - |
Catharanthus roseus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geissoschizine + NADP+ | Catharanthus roseus | involved in interconversion of heteroyohimbine alkaloids, the enzyme channels geissoschizine by a side reaction into the main pathway | 4,21-dehydrogeissoschizine + NADPH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Catharanthus roseus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| geissoschizine + NADP+ = 4,21-didehydrogeissoschizine + NADPH | removal of 21alpha-hydrogen takes place from the less hindered site | Catharanthus roseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Catharanthus roseus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000168 | - |
- |
Catharanthus roseus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 14 days | Catharanthus roseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geissoschizine + NADP+ | structurally related indole alkaloids are not dehydrogenated | Catharanthus roseus | 4,21-dehydrogeissoschizine + NADPH | - |
? | |
| geissoschizine + NADP+ | involved in interconversion of heteroyohimbine alkaloids, the enzyme channels geissoschizine by a side reaction into the main pathway | Catharanthus roseus | 4,21-dehydrogeissoschizine + NADPH | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Catharanthus roseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Catharanthus roseus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | - |
Catharanthus roseus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | NAD+, FAD or FMN cannot act as cofactors | Catharanthus roseus | |
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