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Literature summary for 1.3.1.33 extracted from

  • Apel, K.; Santel, H.J.; Redlinger, T.E.; Falk, H.
    The protochlorophyllide holochrome of barley (Hordeum vulgare L.). Isolation and characterization of the NADPH: protochlorophyllide oxidoreductase (1980), Eur. J. Biochem., 111, 251-258.
    View publication on PubMed
Search for more literature for 1.3.1.33

General Stability

General Stability Organism
active enzyme is protected by NADPH, protochlorophyllide and glycerol Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
etioplast
-
 Hordeum vulgare 9513
-
etioplast prolamellar body
-
 Hordeum vulgare 9541
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 SDS-PAGE Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protochlorophyllide + NADPH Hordeum vulgare
-
 chlorophyllide + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
 barley
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 1.6 nmol chlorophyllide/mg protein/flash Hordeum vulgare

Storage Stability

Storage Stability Organism
0°C, can be kept in elution buffer for at least 3h without any detectable loss of activity Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protochlorophyllide + NADPH
-
 Hordeum vulgare chlorophyllide + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Hordeum vulgare