Cloned (Comment) | Organism |
---|---|
gene macA, DNA sequence determination and analysis, gene cluster analysis, the cluster also comprises genes macB and macR, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, overexpression in Escherichia coli strain BL21(DE3) | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-maleylacetate + NAD(P)H | Cupriavidus necator | enzyme plays a crucial role in the degradation of aromatic compounds | 3-oxoadipate + NAD(P)+ | - |
? | |
2-maleylacetate + NAD(P)H | Cupriavidus necator DSM 531 | enzyme plays a crucial role in the degradation of aromatic compounds | 3-oxoadipate + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | Q9RBG1 | strain 335, gene macA | - |
Cupriavidus necator DSM 531 | Q9RBG1 | strain 335, gene macA | - |
Purification (Comment) | Organism |
---|---|
native enzyme, over 246fold by several chromatographic steps | Cupriavidus necator |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme is required for growth on 4-fluorobenzoate as sole carbon source | Cupriavidus necator | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
above, purified enzyme | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloromaleylacetate + NAD(P)H | - |
Cupriavidus necator | ? + NAD(P)+ | - |
? | |
2-chloromaleylacetate + NAD(P)H | - |
Cupriavidus necator DSM 531 | ? + NAD(P)+ | - |
? | |
2-maleylacetate + NAD(P)H | - |
Cupriavidus necator | 3-oxoadipate + NAD(P)+ | - |
? | |
2-maleylacetate + NAD(P)H | enzyme plays a crucial role in the degradation of aromatic compounds | Cupriavidus necator | 3-oxoadipate + NAD(P)+ | - |
? | |
2-maleylacetate + NAD(P)H | - |
Cupriavidus necator DSM 531 | 3-oxoadipate + NAD(P)+ | - |
? | |
2-maleylacetate + NAD(P)H | enzyme plays a crucial role in the degradation of aromatic compounds | Cupriavidus necator DSM 531 | 3-oxoadipate + NAD(P)+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Cupriavidus necator |