Literature summary for 1.3.1.32 extracted from

Daubaras, D.L.; Saido, K.; Chakrabarty, A.M.
Purification of hydroxyquinol 1,2-dioxygenase and maleylacetate reductase: the lower pathway of 2,4,5-trichlorophenoxyacetic acid metabolism by Burkholderia cepacia AC1100 (1996), Appl. Environ. Microbiol., 62, 4276-4279.

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Cloned(Commentary)

Cloned (Comment)	Organism
gen tftE cloned and overexpressed in Escherichia coli	Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-maleylacetate + NADPH	Pseudomonas sp.	-	3-oxoadipate + NADP+	-	ir
2-maleylacetate + NADPH	Burkholderia cepacia	-	3-oxoadipate + NADP+	-	ir
2-maleylacetate + NADPH	Pseudomonas sp. B13	-	3-oxoadipate + NADP+	-	ir
2-maleylacetate + NADPH	Burkholderia cepacia AC1100	-	3-oxoadipate + NADP+	-	ir

Organism

Organism	UniProt	Comment	Textmining
Burkholderia cepacia	-	AC1100	-
Burkholderia cepacia AC1100	-	AC1100	-
Pseudomonas sp.	-	-	-
Pseudomonas sp. B13	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified from recombinant Escherichia coli cells	Burkholderia cepacia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-maleylacetate + NAD(P)H	-	Pseudomonas sp.	3-oxoadipate + NAD(P)+	-	ir
2-maleylacetate + NAD(P)H	-	Burkholderia cepacia	3-oxoadipate + NAD(P)+	-	ir
2-maleylacetate + NAD(P)H	-	Pseudomonas sp. B13	3-oxoadipate + NAD(P)+	-	ir
2-maleylacetate + NAD(P)H	-	Burkholderia cepacia AC1100	3-oxoadipate + NAD(P)+	-	ir
2-maleylacetate + NADPH	-	Pseudomonas sp.	3-oxoadipate + NADP+	-	ir
2-maleylacetate + NADPH	-	Burkholderia cepacia	3-oxoadipate + NADP+	-	ir
2-maleylacetate + NADPH	-	Pseudomonas sp. B13	3-oxoadipate + NADP+	-	ir
2-maleylacetate + NADPH	-	Burkholderia cepacia AC1100	3-oxoadipate + NADP+	-	ir

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Pseudomonas sp.
NADH	-	Burkholderia cepacia

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Release 2023.1 (January 2023)