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Literature summary for 1.3.1.31 extracted from

  • Wang, H.; Pei, X.; Wu, Z.
    An enoate reductase Achr-OYE4 from Achromobacter sp. JA81: characterization and application in asymmetric bioreduction of CC bonds (2013), Appl. Microbiol. Biotechnol., 98, 705-15.
    View publication on PubMed
Search for more literature for 1.3.1.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Achromobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 2-methyl-N-phenylmaleimide pH 7.5, 30°C Achromobacter sp.
0.0191
-
 NADH pH 7.5, 30°C Achromobacter sp.
0.0854
-
 NADPH pH 7.5, 30°C Achromobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40600
-
 calculated from cDNA Achromobacter sp.
45000
-
 SDS-PAGE Achromobacter sp.

Organism

Organism UniProt Comment Textmining
Achromobacter sp. I3V5V6
-
-
Achromobacter sp. JA81 I3V5V6
-
-

Purification (Commentary)

Purification (Comment) Organism
using affinity chromatography Achromobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-N-phenylmaleimide + NADH + H+
-
 Achromobacter sp. (3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+
-
 ?
2-methyl-N-phenylmaleimide + NADH + H+
-
 Achromobacter sp. JA81 (3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+
-
 ?
2-methyl-N-phenylmaleimide + NADPH
-
 Achromobacter sp. (3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+
-
 ?
2-methyl-N-phenylmaleimide + NADPH
-
 Achromobacter sp. JA81 (3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
Achr-OYE4
-
 Achromobacter sp.
enoate reductase
-
 Achromobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 optimal temperature in Tris-HCl buffer Achromobacter sp.
35
-
 optimal temperature in potassium phosphate buffer Achromobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
 about 40% of maximal activity maintained Achromobacter sp.
45
-
 about 40% of maximal activity maintained Achromobacter sp.
55
-
 no activity beyond 55°C Achromobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 half-life of inactivation: 64.8 h Achromobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.9
-
 2-methyl-N-phenylmaleimide pH 7.5, 30°C Achromobacter sp.
19.2
-
 NADPH pH 7.5, 30°C Achromobacter sp.
38.6
-
 NADH pH 7.5, 30°C Achromobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 highest activity using Tris-HCl Achromobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 no activity at pH 11 Achromobacter sp.
9
-
 only 30% of highest activity at pH 9 Achromobacter sp.

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Achromobacter sp.
NADH preferred cofactor Achromobacter sp.
NADPH
-
 Achromobacter sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4
-
 2-methyl-N-phenylmaleimide pH 7.5, 30°C Achromobacter sp.
220
-
 NADPH pH 7.5, 30°C Achromobacter sp.
2000
-
 NADH pH 7.5, 30°C Achromobacter sp.