Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.31 extracted from

  • Rohdich, F.; Wiese, A.; Feicht, R.; Simon, H.; Bacher, A.
    Enoate reductases of Clostridia: cloning, sequencing, and expression (2001), J. Biol. Chem., 276, 5779-5787.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Clostridium tyrobutyricum
expressed in Escherichia coli growing under anaerobic conditions Moorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
x * 73000, SDS-PAGE Moorella thermoacetica
600000
-
gel filtration Moorella thermoacetica

Organism

Organism UniProt Comment Textmining
Clostridium tyrobutyricum O52933
-
-
Moorella thermoacetica O52935
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Moorella thermoacetica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
-
Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-2-methylbutenoate + NADH
-
Clostridium tyrobutyricum (R)-2-methylbutanoate + NAD+
-
?
(E)-2-methylbutenoate + NADH
-
Moorella thermoacetica (R)-2-methylbutanoate + NAD+
-
?
butenoate + NADH
-
Clostridium tyrobutyricum butanoate + NAD+
-
?
butenoate + NADH
-
Moorella thermoacetica butanoate + NAD+
-
?

Subunits

Subunits Comment Organism
? x * 73000, SDS-PAGE Moorella thermoacetica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Moorella thermoacetica

pH Range

pH Minimum pH Maximum Comment Organism
5 9 active between pH 5.0 and pH 9.0 Moorella thermoacetica

Cofactor

Cofactor Comment Organism Structure
NADH
-
Clostridium tyrobutyricum
NADH
-
Moorella thermoacetica