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Literature summary for 1.3.1.29 extracted from

  • Costa, D.M.; Costa, M.A.; Guimaraes, S.L.; Coitinho, J.B.; Gomez, S.V.; Brandao, T.A.; Nagem, R.A.
    A combined approach for enhancing the stability of recombinant cis-dihydrodiol naphthalene dehydrogenase from Pseudomonas putida G7 allowed for the structural and kinetic characterization of the enzyme (2017), Protein Expr. Purif., 132, 50-59.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M calcium chloride dehydrate, 0.1 M HEPES sodium pH 7.5, 28% (w/v) polyethylene glycol 400 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol at pH 7.0 and 25°C Pseudomonas putida
0.012
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol at pH 9.4 and 25°C Pseudomonas putida
0.31
-
NAD+ at pH 9.4 and 25°C Pseudomonas putida
0.35
-
NAD+ at pH 7.0 and 25°C Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27521
-
-
Pseudomonas putida
122000
-
His6-tagged enzyme, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ Pseudomonas putida
-
naphthalene-1,2-diol + NADH + H+
-
?
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ Pseudomonas putida G7
-
naphthalene-1,2-diol + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q9R9Y8
-
-
Pseudomonas putida G7 Q9R9Y8
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
Pseudomonas putida naphthalene-1,2-diol + NADH + H+
-
?
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
Pseudomonas putida G7 naphthalene-1,2-diol + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 27000, His6-tagged enzyme, SDS-PAGE Pseudomonas putida
homotetramer 4 * 29520, calculated from amino acid sequence Pseudomonas putida

Synonyms

Synonyms Comment Organism
cis-1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase
-
Pseudomonas putida
cis-dihydrodiol naphthalene dehydrogenase
-
Pseudomonas putida
NahB
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.6
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol at pH 7.0 and 25°C Pseudomonas putida
8.3
-
NAD+ at pH 7.0 and 25°C Pseudomonas putida
26.4
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol at pH 9.4 and 25°C Pseudomonas putida
36.6
-
NAD+ at pH 9.4 and 25°C Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida