Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.2 M calcium chloride dehydrate, 0.1 M HEPES sodium pH 7.5, 28% (w/v) polyethylene glycol 400 | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
(1R,2S)-1,2-dihydronaphthalene-1,2-diol | at pH 7.0 and 25°C | Pseudomonas putida | |
0.012 | - |
(1R,2S)-1,2-dihydronaphthalene-1,2-diol | at pH 9.4 and 25°C | Pseudomonas putida | |
0.31 | - |
NAD+ | at pH 9.4 and 25°C | Pseudomonas putida | |
0.35 | - |
NAD+ | at pH 7.0 and 25°C | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27521 | - |
- |
Pseudomonas putida |
122000 | - |
His6-tagged enzyme, gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | Pseudomonas putida | - |
naphthalene-1,2-diol + NADH + H+ | - |
? | |
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | Pseudomonas putida G7 | - |
naphthalene-1,2-diol + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q9R9Y8 | - |
- |
Pseudomonas putida G7 | Q9R9Y8 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
Pseudomonas putida | naphthalene-1,2-diol + NADH + H+ | - |
? | |
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
Pseudomonas putida G7 | naphthalene-1,2-diol + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 27000, His6-tagged enzyme, SDS-PAGE | Pseudomonas putida |
homotetramer | 4 * 29520, calculated from amino acid sequence | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
cis-1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase | - |
Pseudomonas putida |
cis-dihydrodiol naphthalene dehydrogenase | - |
Pseudomonas putida |
NahB | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
(1R,2S)-1,2-dihydronaphthalene-1,2-diol | at pH 7.0 and 25°C | Pseudomonas putida | |
8.3 | - |
NAD+ | at pH 7.0 and 25°C | Pseudomonas putida | |
26.4 | - |
(1R,2S)-1,2-dihydronaphthalene-1,2-diol | at pH 9.4 and 25°C | Pseudomonas putida | |
36.6 | - |
NAD+ | at pH 9.4 and 25°C | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida |