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Literature summary for 1.3.1.29 extracted from
- Bacterial cis-dihydrodiol dehydrogenases: comparison of physicochemical and immunological protperties (1976), J. Bacteriol., 128, 842-850.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
cis-1,2-dihydronaphthalene-1,2-diol | - |
Nocardia sp. |  |
| 0.02 | - |
cis-1,2-dihydronaphthalene-1,2-diol | strain NP | Pseudomonas putida | |
| 0.024 | - |
cis-1,2-dihydronaphthalene-1,2-diol | strain NCIB | Pseudomonas putida | |
| 0.024 | - |
cis-Toluene dihydrodiol | strain biotype A grown on toluene | Pseudomonas putida | |
| 0.05 | - |
cis-benzene dihydrodiol | strain biotype A grown on benzene | Pseudomonas putida | |
| 0.071 | - |
NAD+ | strain biotype A grown on toluene | Pseudomonas putida | |
| 0.079 | - |
NAD+ | strain biotype A grown on benzene | Pseudomonas putida | |
| 0.65 | - |
NAD+ | - |
Nocardia sp. | |
| 1.02 | - |
NAD+ | strain NP | Pseudomonas putida | |
| 1.04 | - |
NAD+ | strain NCIB | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
gel filtration | Nocardia sp. |
| 112000 | - |
strain NCIB 9816, gel filtration | Pseudomonas putida |
| 155000 | 160000 | biotype A, depending on growth medium, gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nocardia sp. | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida | - |
strains NP, PL6 and C84-15 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.19 | - |
activity in crude extracts | Nocardia sp. |
| 0.25 | - |
activity in crude extracts of strain NP | Pseudomonas putida |
| 0.42 | - |
activity in crude extracts of strain NCIB | Pseudomonas putida |
| 0.62 | - |
activity in crude extracts of strain biotype A grown on benzene | Pseudomonas putida |
| 0.94 | - |
activity in crude extracts of strain biotype A grown on toluene | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ | - |
Pseudomonas putida | 1,2-dihydroxynaphthalene + NADH | - |
? | |
| cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ | - |
Nocardia sp. | 1,2-dihydroxynaphthalene + NADH | - |
? | |
| cis-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
Pseudomonas putida | naphthalene-1,2-diol + NADH + H+ | - |
r | |
| cis-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
Nocardia sp. | naphthalene-1,2-diol + NADH + H+ | - |
r | |
| cis-anthracene dihydrodiol + NAD+ | - |
Pseudomonas putida | 1,2-dihydroxyanthracene + NADH | - |
? | |
| cis-anthracene dihydrodiol + NAD+ | - |
Nocardia sp. | 1,2-dihydroxyanthracene + NADH | - |
? | |
| cis-benzene dihydrodiol + NAD+ | - |
Pseudomonas putida | 1,2-dihydroxybenzene + NADH | - |
? | |
| cis-benzene dihydrodiol + NAD+ | - |
Nocardia sp. | 1,2-dihydroxybenzene + NADH | - |
? | |
| cis-biphenyl dihydrodiol + NAD+ | - |
Pseudomonas putida | 3-phenyl-1,2-dihydroxybenzene + NADH | - |
? | |
| cis-biphenyl dihydrodiol + NAD+ | - |
Nocardia sp. | 3-phenyl-1,2-dihydroxybenzene + NADH | - |
? | |
| cis-ethylbenzene dihydrodiol + NAD+ | - |
Pseudomonas putida | 3-ethyl-1,2-dihydroxybenzene + NADH | - |
? | |
| cis-ethylbenzene dihydrodiol + NAD+ | - |
Nocardia sp. | 3-ethyl-1,2-dihydroxybenzene + NADH | - |
? | |
| cis-phenanthrene dihydrodiol + NAD+ | - |
Pseudomonas putida | 3,4-dihydroxyphenanthrene + NADH | - |
? | |
| cis-phenanthrene dihydrodiol + NAD+ | - |
Nocardia sp. | 3,4-dihydroxyphenanthrene + NADH | - |
? | |
| cis-toluene dihydrodiol + NAD+ | - |
Pseudomonas putida | 3-methyl-1,2-dihydroxybenzene + NADH | - |
? | |
| cis-toluene dihydrodiol + NAD+ | - |
Nocardia sp. | 3-methyl-1,2-dihydroxybenzene + NADH | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
in cell extract: Pseudomonas putida NP stable, other Pseudomonas strains and Nocardia sp. unstable | Pseudomonas putida |
| 50 | - |
in cell extract: Pseudomonas putida NP stable, other Pseudomonas strains and Nocardia sp. unstable | Nocardia sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Nocardia sp. |
| 8.8 | 9 | - |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas putida | |
| NAD+ | - |
Nocardia sp. | |
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