Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(-)-epigallocatechin-3-gallate | i.e. EGCG, from Thea sativa, slightly inhibits the liver microsomal enzyme | Rattus norvegicus | |
additional information | theaflavin, gallic acid, and n-propyl gallate are poor inhibitors, physiological effects of inhibitors in vivo, overview | Rattus norvegicus | |
penta-O-galloyl-beta-D-glucose | from Thea sativa, inhibits the expression of androgen receptor and reduce secretion of prostate-specific antigen in LNCaP prostate cancer cells | Homo sapiens | |
penta-O-galloyl-beta-D-glucose | i.e. 5GG, from Thea sativa, inhibits the liver microsomal enzyme | Rattus norvegicus | |
theaflavin-3'-gallate | i.e. TF2b, from Thea sativa, inhibits the liver microsomal enzyme | Rattus norvegicus | |
theaflavin-3,3'-digallate | from Thea sativa, inhibits the expression of androgen receptor and reduce secretion of prostate-specific antigen in LNCaP prostate cancer cells | Homo sapiens | |
theaflavin-3,3'-digallate | i.e. TF3, from Thea sativa, inhibits the liver microsomal enzyme | Rattus norvegicus | |
theaflavin-3-gallate | i.e. TF2a, from Thea sativa, inhibits the liver microsomal enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00245 | - |
testosterone | pH 6.5, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
testosterone + NADPH + H+ | Rattus norvegicus | - |
5alpha-dihydrotestosterone + NADP+ | 5alpha-dihydrotestosterone is a more potent androgen, regulatory function, overview | ? | |
testosterone + NADPH + H+ | Homo sapiens | - |
5alpha-dihydrotestosterone + NADP+ | dihydrotestosterone is a more potent androgen, regulatory function, overview | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
2 isozymes 5alpha-R1 and 5alpha-R2 | - |
Rattus norvegicus | - |
2 isozymes 5alpha-R1 and 5alpha-R2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | only isozyme 5alpha-R1 | Rattus norvegicus | - |
LNCaP cell | prostate cancer cell line | Homo sapiens | - |
prostate | - |
Homo sapiens | - |
prostate | - |
Rattus norvegicus | - |
prostate carcinoma cell | - |
Homo sapiens | - |
prostate carcinoma cell | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
testosterone + NADPH + H+ | - |
Homo sapiens | 5alpha-dihydrotestosterone + NADP+ | - |
? | |
testosterone + NADPH + H+ | - |
Rattus norvegicus | 5alpha-dihydrotestosterone + NADP+ | - |
? | |
testosterone + NADPH + H+ | - |
Rattus norvegicus | 5alpha-dihydrotestosterone + NADP+ | 5alpha-dihydrotestosterone is a more potent androgen, regulatory function, overview | ? | |
testosterone + NADPH + H+ | - |
Homo sapiens | 5alpha-dihydrotestosterone + NADP+ | dihydrotestosterone is a more potent androgen, regulatory function, overview | ? |
Synonyms | Comment | Organism |
---|---|---|
steroid 5alpha-reductase | - |
Homo sapiens |
steroid 5alpha-reductase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |
6.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens | |
NADPH | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Rattus norvegicus | |
0.00036 | - |
theaflavin-3,3'-digallate | pH 6.5, 37°C, versus NADPH | Rattus norvegicus | |
0.00048 | - |
penta-O-galloyl-beta-D-glucose | pH 6.5, 37°C, versus NADPH | Rattus norvegicus | |
0.0026 | - |
theaflavin-3'-gallate | pH 6.5, 37°C, versus NADPH | Rattus norvegicus | |
0.0027 | - |
penta-O-galloyl-beta-D-glucose | pH 6.5, 37°C, versus testosterone | Rattus norvegicus | |
0.0056 | - |
theaflavin-3,3'-digallate | pH 6.5, 37°C, versus testosterone | Rattus norvegicus | |
0.0065 | - |
theaflavin-3'-gallate | pH 6.5, 37°C, versus testosterone | Rattus norvegicus |