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Literature summary for 1.3.1.22 extracted from

  • Moore, R.J.; Wilson, J.D.
    Extraction of the reduced nicotinamide adenine dinucleotide phosphate:delta 4-3-ketosteroid-5-alpha oxidoreductase of rat prostate with digitonin and potassium chloride (1974), Biochemistry, 13, 450-456.
    View publication on PubMed
Search for more literature for 1.3.1.22

General Stability

General Stability Organism
glycerol stabilizes Rattus norvegicus
NADPH stabilizes Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Rattus norvegicus 5783
-
membrane of endoplasmic reticulum Rattus norvegicus 16020
-
nuclear envelope
-
 Rattus norvegicus 5635
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000 35000 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
prostate gland ventral Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1090
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholest-4-en-3-one + NADPH
-
 Rattus norvegicus 5-alpha-cholestan-3-one + NADP+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
-
 Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.7 8.4 in the cold Rattus norvegicus
6.6 7.8 4 days, in the cold, presence of KCl and NADH Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Rattus norvegicus