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Literature summary for 1.3.1.20 extracted from

  • Deyashiki, Y.; Tamada, Y.; Miyabe, Y.; Nakanishi, M.; Matsuura, K.; Hara, A.
    Expression and kinetic properties of a recombinant 3alpha-hydroxysteroid/dihydrodiol dehydrogenase isoenzyme of human liver (1995), J. Biochem., 118, 285-290.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DD4, expression in Escherichia coli JM109 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-Indanone recombinant DD4 Homo sapiens
17beta-estradiol DD4, recombinant enzyme Homo sapiens
3',3'',5',5''-tetrabromophenolphthalein recombinant DD4 Homo sapiens
Betamethasone DD4, recombinant enzyme Homo sapiens
Bromophenol blue recombinant DD4 Homo sapiens
dexamethasone DD4, recombinant enzyme Homo sapiens
Flufenamic acid DD4, recombinant enzyme Homo sapiens
Hexestrol DD4, recombinant enzyme Homo sapiens
indomethacin recombinant DD4 Homo sapiens
Phenol red recombinant DD4 Homo sapiens
Phenolphthalein DD4, recombinant enzyme, inhibition mechanism Homo sapiens
phenolphthalein phosphate recombinant DD4 Homo sapiens
Thymolphthalein recombinant DD4 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
recombinant DD4, gel filtration Homo sapiens
36000
-
DD4, native PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
DD4
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.23
-
recombinant DD4, substrate (S)-indan-1-ol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-indan-1-ol + NADP+
-
Homo sapiens ?
-
?
(S)-indan-1-ol + NADP+
-
Homo sapiens ?
-
?
5alpha-dihydrotestosterone + NADPH + H+
-
Homo sapiens 5alpha-androstan-3alpha,17beta-diol + NADP+
-
?
5beta-androstan-3,17-dione + NADPH
-
Homo sapiens 5beta-androstan-3alpha-ol-17-one + NADP+
-
?
5beta-androstan-3alpha,17beta-diol + NADP+
-
Homo sapiens 5beta-androstan-3,17-dione + NADPH
-
?
5beta-dihydrocortisone + NADPH
-
Homo sapiens ?
-
?
5beta-pregnan-3,20-diol + NADP+
-
Homo sapiens 5beta-pregnan-20-ol-3-one + NADPH
-
?
5beta-pregnan-3,20-dione + NADPH
-
Homo sapiens 3alpha-hydroxy-5beta-pregnan-20-one + NADP+
-
?
7alpha-hydroxy-5beta-cholestan-3-one + NADPH
-
Homo sapiens ?
-
?
androsterone + NADPH
-
Homo sapiens ?
-
?
cholic acid + NADP+
-
Homo sapiens ?
-
?
lithocholic acid + NADP+
-
Homo sapiens ?
-
?
pyridine-3-aldehyde + NADPH
-
Homo sapiens ?
-
?
trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+
-
Homo sapiens catechol + NAD(P)H
-
?
trans-naphthalene dihydrodiol + NAD(P)+
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer 1* 35000, recombinant DD4, 1 * 36000, native DD4, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
DD
-
Homo sapiens
dihydrodiol dehydrogenase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00008
-
Phenolphthalein recombinant DD4 Homo sapiens
0.15
-
1-Indanone recombinant DD4 Homo sapiens