Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sulfide | 8.0 mol acid-labile sulfide per mol of subunit | Sus scrofa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,6-dihydrouracil | - |
Sus scrofa | |
ATP-ribose | dead-end inhibition | Sus scrofa | |
NADP+ | competitive versus NADPH | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
Uracil | - |
Sus scrofa | |
0.006 | - |
NADPH | - |
Sus scrofa | |
0.0066 | - |
NADPH | in presence of 2,6-dihydrouracil | Sus scrofa | |
0.023 | - |
Uracil | in presence of 2,6-dihydrouracil | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 2 [4Fe-4S] clusters per subunit with 9.0 mol iron per mol of subunit | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107000 | - |
2 * 107000, SDS-PAGE | Sus scrofa |
214000 | - |
recombinant from E. coli, native PAGE | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Sus scrofa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14 | - |
recombinant from Escherichia coli | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + NADPH | - |
Sus scrofa | 5,6-dihydrouracil + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 107000, SDS-PAGE | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | contains 2 mol FMN and 2 mol FAD per mol of enzyme, tightly associated | Sus scrofa | |
NADPH | strictly dependent on | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibitory reactions between uracil, NADP+ and dihydrouracil | Sus scrofa |