Literature summary for 1.3.1.2 extracted from

Schmitt, U.; Jahnke, K.; Rosenbaum, K.; Cook, P.F.; Schnackerz, K.D.
Purification and characterization of dihydropyrimidine dehydrogenase from Alcaligenes eutrophus (1996), Arch. Biochem. Biophys., 332, 175-182.

Search for more literature for 1.3.1.2

Activating Compound

Activating Compound	Comment	Organism	Structure
Sulfide	23.4 mol per mol of enzyme	Cupriavidus necator

Inhibitors

Inhibitors	Comment	Organism	Structure
3-aminopyridine ADP	-	Cupriavidus necator
5,6-dihydrouracil	-	Cupriavidus necator
ATP-ribose	dead-end inhibition	Cupriavidus necator
additional information	not: thio-NADPH, cyclo-NADPH, deamino-NADPH	Cupriavidus necator
NADP+	competitive versus NADPH	Cupriavidus necator
NADPH	-	Cupriavidus necator

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0049	-	Uracil	-	Cupriavidus necator
0.0068	-	NADPH	in presence of uracil	Cupriavidus necator

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	iron atoms are probably present in iron-sulfur centers	Cupriavidus necator
Iron	24.5 mol iron per mol of enzyme	Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52000	-	4 * 52000, SDS-PAGE	Cupriavidus necator
207000	210000	gel fitration or gradient native PAGE	Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
uracil + NADPH	Cupriavidus necator	rate limiting step in catabolic breakdown of uracil to beta-alanine	5,6-dihydrouracil + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Cupriavidus necator

Reaction

Reaction	Comment	Organism	Reaction ID
5,6-dihydrouracil + NADP+ = uracil + NADPH + H+	abstraction of pro-S hydrogen of NADPH and thus B-side stereospecific class dehydrogenase	Cupriavidus necator	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
182.1	-	purified enzyme	Cupriavidus necator

Storage Stability

Storage Stability	Organism
-80°C, 35 mM potassium phosphate, pH 7.4, 1 mM dithioerythritol, more than 2 months without loss of activity, without dithioerythritol 50% loss of activity	Cupriavidus necator

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-aminouracil + NADPH	-	Cupriavidus necator	?	-	?
5-fluorouracil + NADPH + H+	-	Cupriavidus necator	5-fluoro-5,6-dihydrouracil + NADP+	-	?
dihydrothymine + NADP+	-	Cupriavidus necator	thymine + NADPH	-	r
additional information	not: orotate, cytosine	Cupriavidus necator	?	-	?
uracil + NADPH	-	Cupriavidus necator	5,6-dihydrouracil + NADP+	-	?
uracil + NADPH	rate limiting step in catabolic breakdown of uracil to beta-alanine	Cupriavidus necator	5,6-dihydrouracil + NADP+	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 52000, SDS-PAGE	Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Cupriavidus necator

Cofactor

Cofactor	Comment	Organism	Structure
flavin	4.1 mol FMN per mole of enzyme	Cupriavidus necator
NADP+	-	Cupriavidus necator
NADPH	-	Cupriavidus necator

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	Ki for ATP-ribose, 3-aminopyridine ADP, and 2,6-dihydrouracil	Cupriavidus necator
additional information	-	additional information	inhibitory reactions between uracil, NADP+ and dihydrouracil	Cupriavidus necator
0.007	-	NADPH	-	Cupriavidus necator
0.011	-	NADP+	-	Cupriavidus necator
0.15	-	dihydrouracil	-	Cupriavidus necator

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Release 2023.1 (January 2023)