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Literature summary for 1.3.1.2 extracted from
- Inactivation of dihydropyrimidine dehydrogenase by 5-iodouracil (1991), J. Biol. Chem., 266, 19988-19994.
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol stabilizes against inactivation via 5-alkylyluracils | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-Iodouracil | dithiothreitol and thymine protect against inactivation | Bos taurus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thymine + NADPH | Bos taurus | rate-limiting enzyme in catabolic detoxification of exogenous fluoropyrimidines | dihydrothymine + NADP+ | - |
? | |
| uracil + NADPH | Bos taurus | - |
5,6-dihydrouracil + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.38 | - |
purified enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorouracil + NADPH + H+ | - |
Bos taurus | 5-fluoro-5,6-dihydrouracil + NADP+ | - |
? | |
| 5-iodouracil + NADPH | - |
Bos taurus | 5-iodo-5,6-dihydrouracil + NADP+ | alkylating and therefore in vivo inactivating product | ? | |
| thymine + NADPH | - |
Bos taurus | dihydrothymine + NADP+ | - |
? | |
| thymine + NADPH | rate-limiting enzyme in catabolic detoxification of exogenous fluoropyrimidines | Bos taurus | dihydrothymine + NADP+ | - |
? | |
| uracil + NADPH | - |
Bos taurus | 5,6-dihydrouracil + NADP+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Bos taurus | |
| NADPH | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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