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Literature summary for 1.3.1.15 extracted from
- Properties of triphosphpyridine nucleotide-linked dihydroorotic dehydrogenase (1962), J. Biol. Chem., 237, 2018-2024.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-Dihydroxy-6-methylpyridine | 7.8 mM, 22% inhibition | Bacteria |  |
| 5-Methylorotic acid | 6 mM, 63% inhibition | Bacteria | |
| Barbituric acid | 2 mM, 60% inhibition | Bacteria | |
| p-chloromercuribenzoate | 0.01 mM, 90% inhibition, 0.001 mM, 30% inhibition | Bacteria | |
| Uracil-5-carboxylic acid | 7.8 mM, 30% inhibition | Bacteria | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
Orotate | - |
Bacteria | |
| 0.2 | - |
NADPH | - |
Bacteria | |
| 0.3 | - |
5-Fluoroorotate | - |
Bacteria | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Mg2+ has no effect | Bacteria |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| orotate + NADPH | Bacteria | - |
(S)-dihydroorotate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteria | - |
gram-negative, non-spore forming obligate aerobe | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacteria |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen or at 4°C | Bacteria |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluoroorotate + NADPH | enzyme exhibits very weak oxidase activity | Bacteria | (S)-5-fluorodihydroorotate + NADP+ | - |
? | |
| orotate + NADPH | - |
Bacteria | (S)-dihydroorotate + NADP+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Bacteria |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
8 min, 81% loss of activity | Bacteria |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.33 | 11.7 | Orotate | - |
Bacteria | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
sodium phosphate buffer | Bacteria |
| 7.7 | - |
Tris buffer | Bacteria |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 8.8 | at pH 6.7 and 8.8: about 25% of activity maximum | Bacteria |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | ratio FAD/FMN 1:1 (probably) | Bacteria | |
| FMN | ratio FAD/FMN 1:1 (probably) | Bacteria | |
| NADPH | - |
Bacteria | |
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Release 2023.1 (January 2023)
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