Protein Variants | Comment | Organism |
---|---|---|
K48A | KM-value for dihydroorotate is 2.2fold higher than wild-type value, kcat is 411fold lower than wild-type value | Lactococcus lactis |
K48E | KM-value for dihydroorotate is 4.1fold higher than wild-type value, kcat is 67.5fold lower than wild-type value | Lactococcus lactis |
K48Q | KM-value for dihydroorotate is 1.5fold higher than wild-type value, kcat is 448fold lower than wild-type value | Lactococcus lactis |
K48R | KM-value for dihydroorotate is 2fold lower than wild-type value, kcat is 117fold lower than wild-type value | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
NADH | wild-type enzyme | Lactococcus lactis | |
0.0144 | - |
Orotate | mutant enzyme K48Q | Lactococcus lactis | |
0.0213 | - |
Orotate | wild-type enzyme | Lactococcus lactis | |
0.044 | - |
(S)-dihydroorotate | mutant enzyme K48R | Lactococcus lactis | |
0.062 | - |
NAD+ | wild-type enzyme | Lactococcus lactis | |
0.09 | - |
(S)-dihydroorotate | wild-type enzyme | Lactococcus lactis | |
0.131 | - |
(S)-dihydroorotate | mutant enzyme K48Q | Lactococcus lactis | |
0.195 | - |
(S)-dihydroorotate | mutant enzyme K48A | Lactococcus lactis | |
0.367 | - |
(S)-dihydroorotate | mutant enzyme K48E | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + NAD+ | - |
Lactococcus lactis | orotate + NADH + H+ | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
NADH | mutant enzyme K48Q | Lactococcus lactis | |
0.021 | - |
Orotate | mutant enzyme K48Q | Lactococcus lactis | |
0.11 | - |
(S)-dihydroorotate | mutant enzyme K48Q | Lactococcus lactis | |
0.12 | - |
(S)-dihydroorotate | mutant enzyme K48A | Lactococcus lactis | |
0.42 | - |
(S)-dihydroorotate | mutant enzyme K48R | Lactococcus lactis | |
0.73 | - |
(S)-dihydroorotate | mutant enzyme K48E | Lactococcus lactis | |
25.7 | - |
NADH | wild-type enzyme | Lactococcus lactis | |
25.7 | - |
Orotate | wild-type enzyme | Lactococcus lactis | |
49.3 | - |
(S)-dihydroorotate | wild-type enzyme | Lactococcus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S-center | - |
Lactococcus lactis | |
FAD | - |
Lactococcus lactis | |
FMN | key charge-stabilizing role for Lys-48 of subunit D during reduction of FMN by dihydroorotate or by electron transfer from the 2Fe-2S center | Lactococcus lactis |