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Literature summary for 1.3.1.12 extracted from

  • Lim, S.; Springstead, J.R.; Yu, M.; Bartkowski, W.; Schroeder, I.; Monbouquette, H.G.
    Characterization of a key trifunctional enzyme for aromatic amino acid biosynthesis in Archaeoglobus fulgidus (2009), Extremophiles, 13, 191-198.
    View publication on PubMed
Search for more literature for 1.3.1.12

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
L-tyrosine 0.5 mM, 50% inhibition Archaeoglobus fulgidus
additional information phenylalanine (up to 5 mM) has no effect. Not inhibited by prephenate or NAD+ at concentrations up to 10 and 2 mM, respectively Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
 NAD+ pH 6.5, 87°C Archaeoglobus fulgidus
0.45
-
 prephenate pH 6.5, 87°C Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 6 * 66000, SDS-PAGE Archaeoglobus fulgidus
70946
-
 6 * 70946, calculated from sequence Archaeoglobus fulgidus
420000
-
 gel filtration Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prephenate + NAD+ Archaeoglobus fulgidus the enzyme is involved in aromatic amino acid biosynthesis 4-hydroxyphenylpyruvate + CO2 + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O30012
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prephenate + NAD+ the enzyme is involved in aromatic amino acid biosynthesis Archaeoglobus fulgidus 4-hydroxyphenylpyruvate + CO2 + NADH
-
 ?
prephenate + NAD+ activity with NADP+ as coenzyme is about 10% of that with NAD+, suggesting that NAD+ is likely the preferred and physiological coenzyme Archaeoglobus fulgidus 4-hydroxyphenylpyruvate + CO2 + NADH
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 66000, SDS-PAGE Archaeoglobus fulgidus
homohexamer 6 * 70946, calculated from sequence Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
AroQ gene name. Trifunctional enzyme EC 5.4.99.5 (chorismate mutase)/EC 4.2.1.51 (prephenate dehydratase)/EC 1.3.1.12 (prephenate dehydrogenase) Archaeoglobus fulgidus
CM/PDT/PDHG trifunctional enzyme EC 5.4.99.5 (chorismate mutase)/EC 4.2.1.51 (prephenate dehydratase)/EC 1.3.1.12 (prephenate dehydrogenase) Archaeoglobus fulgidus
PDHG
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
87
-
 assay at Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
NAD+ activity with NADP+ as coenzyme is about 10% of that with NAD+, suggesting that NAD+ is likely the preferred and physiological coenzyme Archaeoglobus fulgidus

General Information

General Information Comment Organism
metabolism the enzyme is involved in aromatic amino acid biosynthesis Archaeoglobus fulgidus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
880
-
 prephenate pH 6,5, 87°C Archaeoglobus fulgidus