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Literature summary for 1.3.1.11 extracted from

  • Levy, C.C.; Weinstein, G.D.
    The metabolism of coumarin by a microorganism. II. The reduction of o-coumaric acid to melilotic acid (1964), Biochemistry, 3, 1944-1947.
    View publication on PubMed
Search for more literature for 1.3.1.11

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate 3.3 mM, 29% inhibition Arthrobacter sp.
N-ethylmaleimide 3.0 mM, 63% inhibition Arthrobacter sp.
p-chloromercuribenzoate 3.0 mM, 100% inhibition, 0.33 mM, 37% inhibition Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0173
-
 o-coumarate
-
 Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-coumarate + NADH Arthrobacter sp.
-
 melilotate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.56
-
-
 Arthrobacter sp.

Storage Stability

Storage Stability Organism
1 week, -20°C Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dihydroxycinnamic acid + NADH 1.6% of activity with 2-coumarate Arthrobacter sp. ?
-
 ?
2-coumarate + NADH
-
 Arthrobacter sp. melilotate + NAD+
-
 ?
2-coumarate + NADH
-
 Arthrobacter sp. melilotate + NAD+
-
 ir
4-coumarate + NADH 1.4% of activity with 2-coumarate Arthrobacter sp. ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 8.5 o-coumarate + NADH Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arthrobacter sp.