Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28200 | - |
1 * 42500 (electron transfer flavoprotein alpha subunit CarE) + 1 * 41400 (caffeoyl-CoA reductase CarC) + 1 * 28200 (electron transfer flavoprotein beta subunit CarD), SDS-PAGE | Acetobacterium woodii |
41400 | - |
1 * 42500 (electron transfer flavoprotein alpha subunit CarE) + 1 * 41400 (caffeoyl-CoA reductase CarC) + 1 * 28200 (electron transfer flavoprotein beta subunit CarD), SDS-PAGE | Acetobacterium woodii |
42500 | - |
1 * 42500 (electron transfer flavoprotein alpha subunit CarE) + 1 * 41400 (caffeoyl-CoA reductase CarC) + 1 * 28200 (electron transfer flavoprotein beta subunit CarD), SDS-PAGE | Acetobacterium woodii |
350000 | - |
caffeoyl-CoA reductase and the electron transfer flavoprotein CarDE form a stable complex | Acetobacterium woodii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster | Acetobacterium woodii | the caffeoyl-CoA reductase-Etf complex of Acetobacterium woodii uses the mechanism of flavin-dependent electron bifurcation to drive the endergonic ferredoxin reduction with NADH as reductant by coupling it to the exergonic NADH-dependent reduction of caffeoyl-CoA | 3-(3,4-dihydroxyphenyl)propanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster | Acetobacterium woodii DAM 1030 | the caffeoyl-CoA reductase-Etf complex of Acetobacterium woodii uses the mechanism of flavin-dependent electron bifurcation to drive the endergonic ferredoxin reduction with NADH as reductant by coupling it to the exergonic NADH-dependent reduction of caffeoyl-CoA | 3-(3,4-dihydroxyphenyl)propanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacterium woodii | H6LGM6 and H6LGM7 and H6LGM8 | H6LGM6: caffeoyl-CoA reductase CarC, H6LGM7: electron transfer flavoprotein beta subunit CarD, H6LGM8: electron transfer flavoprotein alpha subunit CarE | - |
Acetobacterium woodii DAM 1030 | H6LGM6 and H6LGM7 and H6LGM8 | H6LGM6: caffeoyl-CoA reductase CarC, H6LGM7: electron transfer flavoprotein beta subunit CarD, H6LGM8: electron transfer flavoprotein alpha subunit CarE | - |
Purification (Comment) | Organism |
---|---|
- |
Acetobacterium woodii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster | the caffeoyl-CoA reductase-Etf complex of Acetobacterium woodii uses the mechanism of flavin-dependent electron bifurcation to drive the endergonic ferredoxin reduction with NADH as reductant by coupling it to the exergonic NADH-dependent reduction of caffeoyl-CoA | Acetobacterium woodii | 3-(3,4-dihydroxyphenyl)propanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster | the caffeyl-CoA reductase-Etf complex of Acetobacterium woodii uses the mechanism of flavin-dependent electron bifurcation to drive the endergonic ferredoxin reduction with NADH as reductant by coupling it to the exergonic NADH-dependent reduction of caffeoyl-CoA. Reduction of caffeoyl-CoA is also catalyzed in absence of ferredoxin | Acetobacterium woodii | 3-(3,4-dihydroxyphenyl)propanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster | the caffeoyl-CoA reductase-Etf complex of Acetobacterium woodii uses the mechanism of flavin-dependent electron bifurcation to drive the endergonic ferredoxin reduction with NADH as reductant by coupling it to the exergonic NADH-dependent reduction of caffeoyl-CoA | Acetobacterium woodii DAM 1030 | 3-(3,4-dihydroxyphenyl)propanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster | the caffeyl-CoA reductase-Etf complex of Acetobacterium woodii uses the mechanism of flavin-dependent electron bifurcation to drive the endergonic ferredoxin reduction with NADH as reductant by coupling it to the exergonic NADH-dependent reduction of caffeoyl-CoA. Reduction of caffeoyl-CoA is also catalyzed in absence of ferredoxin | Acetobacterium woodii DAM 1030 | 3-(3,4-dihydroxyphenyl)propanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
4-coumaroyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster | - |
Acetobacterium woodii | ? + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
4-coumaroyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster | - |
Acetobacterium woodii DAM 1030 | ? + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
feruloyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster | - |
Acetobacterium woodii | ? + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
feruloyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster | - |
Acetobacterium woodii DAM 1030 | ? + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 1 * 42500 (electron transfer flavoprotein alpha subunit CarE) + 1 * 41400 (caffeoyl-CoA reductase CarC) + 1 * 28200 (electron transfer flavoprotein beta subunit CarD), SDS-PAGE | Acetobacterium woodii |
Synonyms | Comment | Organism |
---|---|---|
caffeoyl-CoA reductase-Etf complex | - |
Acetobacterium woodii |
electron-bifurcating caffeoyl-CoA reductase | - |
Acetobacterium woodii |
hydrocaffeoyl-CoA:NAD+, ferredoxin oxidoreductase | - |
Acetobacterium woodii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
caffeoyl-CoA-dependent methyl viologen oxidation assay | Acetobacterium woodii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
caffeoyl-CoA-dependent methyl viologen oxidation assay | Acetobacterium woodii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 30% of maximal activity, pH 9.0: about 30% of maximal activity | Acetobacterium woodii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains 4 mol of FAD per of enzyme. The CarCDE complex requires FAD for catalytic activity. No activity can be detected when FAD or FMN (0.25 mM) are used alone as electron acceptor, but together with caffeyl-CoA, they are completely reduced with NADH as reductant. Caffeyl-CoA reduction is stimulated by 26- and 12fold with FAD and FMN, respectively. The Km value for both FAD and FMN is 0.1 mM. Thus, caffeyl-CoA and flavins are reduced simultaneously | Acetobacterium woodii | |
FMN | no activity can be detected when FAD or FMN (0.25 mM) are used alone as electron acceptor, but together with caffeyl-CoA, they are completely reduced with NADH as reductant. Caffeyl-CoA reduction is stimulated by 26- and 12-fold with FAD and FMN, respectively. The Km value for both FAD and FMN is 0.1 mM. Thus, caffeyl-CoA and flavins are reduced simultaneously | Acetobacterium woodii | |
[4Fe-4S]-center | the enzyme contains 2 [4Fe-4S] clusters. It contains 9 mol of iron, and 9 mol of acid-labile sulfur per mol of enzyme | Acetobacterium woodii |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in caffeate respiration | Acetobacterium woodii |