Literature summary for 1.3.1.104 extracted from

Heath, R.J.; Li, J.; Roland, G.E.; Rock, C.O.
Inhibition of the Staphylococcus aureus NADPH-dependent enoyl-acyl carrier protein reductase by triclosan and hexachlorophene (2000), J. Biol. Chem., 275, 4654-4659.

Search for more literature for 1.3.1.104

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Staphylococcus aureus

Protein Variants

Protein Variants	Comment	Organism
G23S	mutation increases the resistance of Staphylococcus aureus to triclosan by an order of magnitude	Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
Hexachlorophene	-	Staphylococcus aureus
triclosan	the introduction of a plasmid expressing the enzyme gene leads to triclosan resistance	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
8	-	crotonyl-N-acetylcysteamine	pH 7.5, 25°C	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	Q9RMI3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
crotonyl-N-acetylcysteamine + NADPH + H+	-	Staphylococcus aureus	butyryl-N-acetylcysteamine + NADP+	-	?
trans-2-octenoyl-N-acetylcysteamine + NADPH + H+	-	Staphylococcus aureus	octanoyl-N-acetylcysteamine + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
FabI	-	Staphylococcus aureus

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	exhibits specific and positive cooperative binding of NADPH. The cofactor concentration that yields 50% of the maximal rate is 65 microM	Staphylococcus aureus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.003	-	pH 7.5, 25°C	Staphylococcus aureus	triclosan

General Information

General Information	Comment	Organism
physiological function	expression complements the Escherichia coli fabI temperature-sensitive mutant	Staphylococcus aureus

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Release 2023.1 (January 2023)