KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km-values for the (S)-alcohols are one order of magnitude lower than those for the (R)-alcohols, indicating that the enzyme has a higher affinity for the S-configuration than for the R-configuration | Nicotiana tabacum | |
1.9 | - |
(2S,4R)-carveol | pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
2.5 | - |
(2S,4S)-carveol | pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
38 | - |
(2R,4R)-carveol | pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
52 | - |
(2R,4S)-carveol | pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
590 | - |
(1R,2S,4S)-neoisodihydrocarveol | pH 8.0, 35°C, NADP+ | Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
alpha2, 2 * 37000, determined by SDS-PAGE | Nicotiana tabacum |
38080 | - |
allyl-ADH1, calculated from sequence | Nicotiana tabacum |
74000 | - |
approximately, gel filtration chromatography on a TSK G3000SW HPLC column | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q9SLN8 | - |
- |
Purification (Comment) | Organism |
---|---|
by DEAE-Toyopearl anion exchange column chromatography, followed by Red-Toyopearl affinity column chromatography | Nicotiana tabacum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
Red-Toyopearl affinity column chromatography, pH 8.0, 35°C | Nicotiana tabacum |
4.6 | - |
DEAE-Toyopearl anion exchange column chromatography, pH 8.0, 35°C | Nicotiana tabacum |
5300 | - |
crude extract, pH 8.0, 35°C | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R,2S,4S)-neoisodihydrocarveol + NADP+ | - |
Nicotiana tabacum | (1R,4S)-isodihydrocarvone + NADPH + H+ | - |
r | |
(2R,4R)-carveol + NADP+ | - |
Nicotiana tabacum | (R)-carvone + NADPH + H+ | - |
r | |
(2R,4S)-carveol + NADP+ | - |
Nicotiana tabacum | (S)-carvone + NADPH + H+ | - |
r | |
(2S,4R)-carveol + NADP+ | - |
Nicotiana tabacum | (R)-carvone + NADPH + H+ | - |
r | |
(2S,4S)-carveol + NADP+ | - |
Nicotiana tabacum | (S)-carvone + NADPH + H+ | - |
r | |
additional information | results indicate that the enzyme catalyzes specifically the dehydrogenation of the secondary alcohols adjacent to the C-C double bond, no oxidation of saturated alcohols | Nicotiana tabacum | ? | - |
? | |
additional information | the enzymatic reaction is a reversible oxidoreduction and the reaction is enantioselective | Nicotiana tabacum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | alpha2, 2 * 37000, determined by SDS-PAGE | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
allyl-ADH | - |
Nicotiana tabacum |
allyl-ADH1 | the amino acid sequences of the deduced allyl-ADHs are different in four amino acid residues | Nicotiana tabacum |
allyl-ADH2 | the amino acid sequences of the deduced allyl-ADHs are different in four amino acid residues, allyl-ADH2 has the following mutations compared to ADH1: T17A, V30A, L345P,R278H | Nicotiana tabacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
(1R,2S,4S)-neoisodihydrocarveol | dehydrogenation, pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
0.003 | - |
(1R,4S)-isodihydrocarvone | hydrogenation to (1R,2S,4S)-neoisodihydrocarveol, pH 7.4, 35°C, NADP+ | Nicotiana tabacum | |
0.01 | - |
(S)-carvone | hydrogenation to (2R,4S)-carveol, pH 7.4, 35°C, NADP+ | Nicotiana tabacum | |
0.012 | - |
(R)-carvone | hydrogenation to (2R,4R)-carveol, pH 7.4, 35°C, NADP+ | Nicotiana tabacum | |
0.02 | - |
(2R,4R)-carveol | dehydrogenation, pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
0.025 | - |
(2R,4S)-carveol | dehydrogenation, pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
0.025 | - |
(S)-carvone | hydrogenation to (2S,4S)-carveol, pH 7.4, 35°C, NADP+ | Nicotiana tabacum | |
0.032 | - |
(R)-carvone | hydrogenation to (2S,4R)-carveol, pH 7.4, 35°C, NADP+ | Nicotiana tabacum | |
0.077 | - |
(2S,4S)-carveol | dehydrogenation, pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
0.097 | - |
(2S,4R)-carveol | dehydrogenation, pH 8.0, 35°C, NADP+ | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
reduction of the carbonyl group of (S)-carvone | Nicotiana tabacum |
8 | - |
dehydrogenation of (2S,4S)-carveol | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | utilization of NADP+ by the enzyme is four times greater than for NAD+ | Nicotiana tabacum | |
NADP+ | utilization of NADP+ by the enzyme is four times greater than for NAD+ | Nicotiana tabacum |