Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JP1111 cells | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
M99T | the mutant is resistant toward inhibition by AFN-1252 | Staphylococcus aureus |
Y147F | the mutant is catalytically defective but retains its sensitivity to inhibitor AFN-1252 | Staphylococcus aureus |
Y147H | the mutant is catalytically defective but retains its sensitivity to inhibitor AFN-1252 | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AFN-1252 | - |
Staphylococcus aureus | |
triclosan | - |
Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus RN4220 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + NADPH + H+ | - |
Staphylococcus aureus | ? | - |
? | |
crotonyl-CoA + NADPH + H+ | - |
Staphylococcus aureus RN4220 | ? | - |
? | |
crotonyl-[acyl-carrier protein] + NADPH + H+ | - |
Staphylococcus aureus | ? | - |
? | |
crotonyl-[acyl-carrier protein] + NADPH + H+ | - |
Staphylococcus aureus RN4220 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enoyl-[acyl-carrier-protein] reductase | - |
Staphylococcus aureus |
FabI | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
crotonyl-[acyl-carrier protein] | mutant enzyme Y147F, pH and temperature not specified in the publication | Staphylococcus aureus | |
0.038 | - |
crotonyl-[acyl-carrier protein] | mutant enzyme Y147H, pH and temperature not specified in the publication | Staphylococcus aureus | |
0.51 | - |
crotonyl-[acyl-carrier protein] | wild type enzyme, pH and temperature not specified in the publication | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Staphylococcus aureus | |
NADPH | - |
Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000004 | - |
AFN-1252 | wild type enzyme, pH and temperature not specified in the publication | Staphylococcus aureus | |
0.000069 | - |
AFN-1252 | mutant enzyme M99T, pH and temperature not specified in the publication | Staphylococcus aureus |