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Literature summary for 1.3.1.10 extracted from

  • Hevener, K.E.; Mehboob, S.; Boci, T.; Truong, K.; Santarsiero, B.D.; Johnson, M.E.
    Expression, purification and characterization of enoyl-ACP reductase II, FabK, from Porphyromonas gingivalis (2012), Protein Expr. Purif., 85, 100-108.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
NH4Cl required for stability and activity. Assay in presence of 0.1 M NH4Cl Porphyromonas gingivalis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Porphyromonas gingivalis

General Stability

General Stability Organism
enzyme can be concentrated to 20 mg/ml without precipitation Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
NADPH
-
Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0171
-
NADPH pH 7.5, 25°C Porphyromonas gingivalis
0.0478
-
crotonyl-[acyl-carrier protein] pH 7.5, 25°C Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33090
-
x * 33090, calculated Porphyromonas gingivalis

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl sulfoxide up to 10%, no loss of activity Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis Q7MAW0
-
-
Porphyromonas gingivalis W83 Q7MAW0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Porphyromonas gingivalis

Storage Stability

Storage Stability Organism
-20°C, 30% glycerol, no significant loss of activity for at least 1 month Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-[acyl-carrier protein] + NADPH + H+
-
Porphyromonas gingivalis butyryl-[acyl-carrier protein] + NADP+
-
?
crotonyl-[acyl-carrier protein] + NADPH + H+
-
Porphyromonas gingivalis W83 butyryl-[acyl-carrier protein] + NADP+
-
?
additional information enzyme possesses intrinsic NADPH oxidase activity in the absence of the crotonyl-CoA substrate Porphyromonas gingivalis ?
-
?
additional information enzyme possesses intrinsic NADPH oxidase activity in the absence of the crotonyl-CoA substrate Porphyromonas gingivalis W83 ?
-
?

Subunits

Subunits Comment Organism
? x * 33090, calculated Porphyromonas gingivalis

Synonyms

Synonyms Comment Organism
enoyl-ACP-reductase II
-
Porphyromonas gingivalis
FabK
-
Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Porphyromonas gingivalis

Cofactor

Cofactor Comment Organism Structure
FMN
-
Porphyromonas gingivalis
NADH poor cofactor Porphyromonas gingivalis
NADPH inhibitory at high concentration Porphyromonas gingivalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.109
-
NADPH pH 7.5, 25°C Porphyromonas gingivalis