Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method with 0.04 M MgCl2, 0.05 M sodium cacodylate pH 6.0 and 11% (v/v) 2-methyl-2,4-pentanediol at 4°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis 168 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-chelated Hi-Trap column chromatography, Hi-Trap Blue HP column chromatography, Q-Sepharose column chromatography, and Superdex-75 gel filtration | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-[acyl-carrier protein] + NADP+ | - |
Bacillus subtilis | trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH + H+ | - |
? | |
acyl-[acyl-carrier protein] + NADP+ | - |
Bacillus subtilis 168 | trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enoyl-ACP reductase III | - |
Bacillus subtilis |
FabL | - |
Bacillus subtilis |
trans-enoyl-[acyl-carrier-protein] reductase | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent | Bacillus subtilis |